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Development of the Otic (Inner Ear) Organoid with Exploring Differentiation-Promoting Factors
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 정, 연훈 | - |
| dc.contributor.author | 김, 한태 | - |
| dc.date.accessioned | 2023-11-16T05:43:58Z | - |
| dc.date.available | 2023-11-16T05:43:58Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/26863 | - |
| dc.language.iso | en | - |
| dc.title | Development of the Otic (Inner Ear) Organoid with Exploring Differentiation-Promoting Factors | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000032820 | - |
| dc.subject.keyword | Otic organoid | - |
| dc.subject.keyword | Inner ear organoid | - |
| dc.subject.keyword | Hair cell proliferation | - |
| dc.subject.keyword | Stem cell | - |
| dc.subject.keyword | Notch signaling | - |
| dc.subject.keyword | Wnt signaling | - |
| dc.description.degree | Doctor | - |
| dc.contributor.department | 대학원 의학과 | - |
| dc.contributor.affiliatedAuthor | 김, 한태 | - |
| dc.date.awarded | 2023 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2023 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
| dc.description.tableOfContents | I. INTRODUCTION 1
II. STUDY 1: Otic Organoid Generation 19 1. Summary of the previous protocol for the generation 19 2. Materials and methods 20 2-1. Maintenance of mESCs 20 2-2. Development of otic organoids 20 2-3. Immunohistochemistry 24 3. Results 24 3-1. Cell aggregates 24 3-2. Hair cell-like cells 27 3-3. Stereocilia and other microstructures 27 III. STUDY 2: Exploration of the Factors 30 1. Finding the promoting factors 30 2. Materials and methods 30 2-1. Maintenance of mESCs and the development 30 2-2. Treatment of DAPT and CHIR-99021 30 2-3. Immunohistochemistry 31 2-4. qRT-PCR 31 3. Results 33 3-1. Treatment between D8 and D12 33 3-2. Treatment between D12 and D18 35 3-3. Treatment between D28 and D34 35 IV. DISCUSSION 38 1. Development of mature otic organoids 38 2. Application of DAPT and CHIIR-99021 to otic organoids 41 3. Application and future direction of otic organoids 44 3-1. Otic organoids as cell therapy 44 3-2. Inner ear organoid-on-a-chip 44 3-3. Otic organoid vesus cochlear implantation 45 V. REFERENCES 48 | - |
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