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Novel treatment combining plasma–activated water for skin wound regeneration by regulation of redox metabolism
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | 이, 혜란 | - |
| dc.date.accessioned | 2023-11-16T05:43:59Z | - |
| dc.date.available | 2023-11-16T05:43:59Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/26864 | - |
| dc.language.iso | en | - |
| dc.title | Novel treatment combining plasma–activated water for skin wound regeneration by regulation of redox metabolism | - |
| dc.title.alternative | 플라즈마수를 결합한 새로운 치료법의 산화 환원 대사 조절을 통한 피부창상 재생 촉진 효과 분석 | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000032932 | - |
| dc.subject.keyword | Plasma-activated water | - |
| dc.subject.keyword | Silk-based biomaterials | - |
| dc.subject.keyword | Redox species | - |
| dc.subject.keyword | Cell migration | - |
| dc.subject.keyword | Wound regeneration | - |
| dc.subject.keyword | Selective cytotoxicity | - |
| dc.subject.keyword | 플라즈마수 | - |
| dc.subject.keyword | 실크-기반 생체재료 | - |
| dc.subject.keyword | 산화환원종 | - |
| dc.subject.keyword | 세포이동 | - |
| dc.subject.keyword | 창상치유 | - |
| dc.subject.keyword | 선택적 세포독성 | - |
| dc.description.degree | Doctor | - |
| dc.contributor.department | 대학원 의학과 | - |
| dc.contributor.affiliatedAuthor | 이, 혜란 | - |
| dc.date.awarded | 2023 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2023 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
| dc.description.tableOfContents | Introduction 1
Materials and Methods 4 A. PAW generation by microwave plasma system 4 B. Measurement of PAW conductivity 5 C. Cell culture 5 D. Cell viability of normal skin cells 6 E. Cell migration assay 6 F. Gelatin zymogram assay 7 G. Quantitative RT-PCR 7 H. Western blot 7 I. Immunocytochemistry 8 J. Measurement of intracellular ROS 9 K. Mitochondrial membrane potential assay (∆Ψm assay) 9 L. Transmission electron microscopy 9 M. In vivo skin wound regeneration study with murine model 10 N. Hematoxylin and eosin staining 11 O. Immunohistochemistry staining 11 P. Solid agar plate assay 11 Q. Extracellular RONS analysis 12 R. Ion chromatography and Griess assay 13 S. Production of PAW-treated silk–fibrin (S-F) composite hydrogel 13 T. Cell viability and morphology analysis on PAW-treated S-F hydrogel 14 U. Microstructure analysis of PAW-treated S-F composite gel 15 V. Tensile property and elasticity assessment of PAW-treated S-F hydrogel 15 W. Analysis of nitric oxide release and degradation of PAW-treated S-F hydrogel 16 X. Assessment of in vitro ECM synthesis on S-F hydrogel 16 Y. Irradiated wound regeneration study by S-F hydrogel treated murine model 17 Z. Immunofluorescent staining analysis of restored radiated wound tissue 18 Results 19 A. Physicochemical properties of PAW by generation of reactive species 19 B. The mortality of normal skin cells was not induced by PAW treatment 20 C. The enhanced cellular migration by PAW was confirmed with increased MMP-2 expressions 21 D. PAW promotes cell migration by modulating focal adhesion molecules 23 E. PAW promotes intracellular ROS signaling-dependent cell migration with mitochondrial activity 25 F. ROS scavenger undermines the PAW-induced promotion of keratinocyte migration 27 G. PAW improves acute murine wound regeneration by promoting skin cell migration 29 H. Sterilization effects of PAW 32 I. The major extracellular effector of PAW was the NO3− isomer 32 J. Treatment of PAW-applied silk-fibrin hydrogel resulted in selective cytotoxicity on both normal and cancer cells 35 K. Surface modification of silk-fibrin hydrogel by PAW treatment improved material properties and biocompatibility 37 L. PAW treatment promoted extracellular matrix accumulation of cells on silk–fibrin hydrogel 39 M. PAW-treated silk-fibrin composite gel improved the regeneration of radiated skin wounds in vivo 41 N. Neovascularization and the anti-inflammatory effect of the PAW-treated silk-fibrin composite gel in radiation wounds of murine skin 43 Discussion 46 References 57 | - |
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