189 345

Cited 81 times in

Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both.

Authors
Nakao, A; Kaczorowski, DJ; Wang, Y; Cardinal, JS; Buchholz, BM; Sugimoto, R; Tobita, K; Lee, S; Toyoda, Y; Billiar, TR; McCurry, KR
Citation
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, 29(5):544-553, 2010
Journal Title
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
ISSN
1053-24981557-3117
Abstract
BACKGROUND: Recent advances in novel medical gases, including hydrogen and carbon monoxide (CO), have demonstrated significant opportunities for therapeutic use. This study was designed to evaluate the effects of inhaled hydrogen or CO, or both, on cold ischemia/reperfusion (I/R) injury of the myocardium.



METHODS: Syngeneic heterotopic heart transplantation was performed in rats after 6 or 18 hours of cold ischemia in Celsior solution. Survival, morphology, apoptosis and marker gene expression were assessed in the grafts after in vivo inhalation of hydrogen (1% to 3%), CO (50 to 250 ppm), both or neither. Both donors and recipients were treated for 1 hour before and 1 hour after reperfusion.



RESULTS: After 6-hour cold ischemia, inhalation of hydrogen (>2%) or CO (250 ppm) alone attenuated myocardial injury. Prolonged cold ischemia for 18 hours resulted in severe myocardial injury, and treatment with hydrogen or CO alone failed to demonstrate significant protection. Dual treatment with hydrogen and CO significantly attenuated I/R graft injury, reducing the infarcted area and decreasing in serum troponin I and creatine phosphokinase (CPK). Hydrogen treatment alone significantly reduced malondialdehyde levels and serum high-mobility group box 1 protein levels as compared with air-treated controls. In contrast, CO only marginally prevented lipid peroxidation, but it suppressed I/R-induced mRNA upregulation for several pro-inflammatory mediators and reduced graft apoptosis.



CONCLUSIONS: Combined therapy with hydrogen and CO demonstrated enhanced therapeutic efficacy via both anti-oxidant and anti-inflammatory mechanisms, and may be a clinically feasible approach for preventing cold I/R injury of the myocardium.
MeSH terms
Administration, InhalationAnimalsApoptosis/drug effectsBlotting, WesternCarbon Monoxide/pharmacology*Cold Ischemia*Drug Therapy, CombinationHeart Transplantation/pathology*Hydrogen/pharmacology*In Situ Nick-End LabelingInflammation Mediators/analysisMacrophages/pathologyMaleMalondialdehyde/analysisMyocardial Reperfusion InjuryMyocardium/pathologyRatsRats, Inbred LewSystemic Inflammatory Response Syndrome/pathologyTransplantation, Heterotopic*
DOI
10.1016/j.healun.2009.10.011
PMID
20036162
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Thoracic & Cardiovascular Surgery
AJOU Authors
이, 성수
Full Text Link
Files in This Item:
Full-Text Not Available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse