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Loss of SM22 is a characteristic signature of colon carcinogenesis and its restoration suppresses colon tumorigenicity in vivo and in vitro.

Authors
Yeo, M; Park, HJ; Kim, DK; Kim, YB; Cheong, JY; Lee, KJ; Cho, SW
Citation
Cancer, 116(11):2581-2589, 2010
Journal Title
Cancer
ISSN
0008-543X1097-0142
Abstract
BACKGROUND: We previously found the down-expression of SM22 in an experimental animal model of colorectal cancer by performing a proteomic analysis. In this study, we addressed the expression and molecular mechanisms of SM22 in human colorectal cancer.



METHODS: To evaluate the expression of SM22 in colon cancers, Western blot and immunohistochemistry were performed in 13 normal, 14 adenomas, and 44 adenocarcinomas. To address the role of SM22 in colon carcinogenesis, SM22 was restored in the colon cancer cells by the transfection with the pIRES2 vector containing full-length SM22 cDNA and tested for tumorigenicity in vivo and in vitro.



RESULTS: SM22 was found to be significantly down-regulated in adenocarcinoma (58%) compared with adenoma (21.4%) and normal (15.3%). The loss of SM22 correlated with poor differentiation of tumor (P = 0.009) and lymph node metastasis (P = 0.029). Restoration of SM22 expression inhibited cell migration, colony-forming ability of cancer cells, and induced retardation of in vivo tumor growth in a xenograft model.



CONCLUSIONS: Loss of SM22 is a molecular signature of colon cancer and is closely associated with progression, differentiation, and metastasis of colon cancer. The restoration of SM22 leads to an inhibition of colon carcinogenesis in vivo and in vitro, suggesting that SM22 might potentially function as a novel tumor suppressor.
MeSH terms
Adenocarcinoma/metabolismAdenoma/metabolismAnimalsCell DifferentiationCell Line, TumorColorectal Neoplasms/*metabolism/pathologyDown-RegulationGenes, Tumor SuppressorHumansLymphatic MetastasisMiceMice, NudeMicrofilament Proteins/*metabolismMuscle Proteins/*metabolismNeoplasm TransplantationTransfection
DOI
10.1002/cncr.25003
PMID
20336793
Appears in Collections:
Journal Papers > Research Organization > Genomic Research Center for Gastroenterology
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
AJOU Authors
여, 말희김, 영배정, 재연이, 광재조, 성원
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