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Superior myocardial preservation with HTK solution over Celsior in rat hearts with prolonged cold ischemia.

DC Field Value Language
dc.contributor.authorLee, S-
dc.contributor.authorHuang, CS-
dc.contributor.authorKawamura, T-
dc.contributor.authorShigemura, N-
dc.contributor.authorStolz, DB-
dc.contributor.authorBilliar, TR-
dc.contributor.authorLuketich, JD-
dc.contributor.authorNakao, A-
dc.contributor.authorToyoda, Y-
dc.date.accessioned2011-05-31T05:21:32Z-
dc.date.available2011-05-31T05:21:32Z-
dc.date.issued2010-
dc.identifier.issn0039-6060-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/2756-
dc.description.abstractBACKGROUND: Increasing allograft ischemic time is a significant risk factor for mortality following heart transplantation (HTx). The purpose of this study was to evaluate the protective effects of histidine-tryptophan-ketoglutarate (HTK) and Celsior (CEL) using a rat HTx model with prolonged cold storage.



METHODS: The hearts were excised from donor rats, stored in cold preservation solution for either 6 or 18 hours, and heterotopically transplanted into syngeneic recipients. Serum creatine phosphokinase (CPK), serum troponin I, graft-infiltrating cells, graft mRNA levels for inflammatory mediators, and tissue adenosine triphosphate (ATP) levels were analyzed, as markers of graft injury.



RESULTS: The recipients of grafts stored in HTK for 18 hours of prolonged cold ischemia had lower levels of serum CPK and tissue malondialdehyde, less upregulation of the mRNAs for IL-6 and inducible nitric oxide synthase, less apoptosis, and higher ATP levels than those receiving grafts stored in CEL and Saline. Cardiac contraction 3 hours after reperfusion was observed in 43% of the cardiac grafts stored in HTK for 18 hours, while no cardiac wall movement was seen in grafts stored in either saline or CEL.



CONCLUSION: Cold storage in HTK exhibited superior protective effects against prolonged cold ischemia in a syngeneic rat transplantation model.
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dc.language.isoen-
dc.subject.MESHAMP-Activated Protein Kinases/metabolism-
dc.subject.MESHAdenosine Triphosphate/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis-
dc.subject.MESHCold Ischemia-
dc.subject.MESHCreatine Kinase/blood-
dc.subject.MESHDisaccharides-
dc.subject.MESHElectrolytes-
dc.subject.MESHGlucose-
dc.subject.MESHGlutamates-
dc.subject.MESHGlutathione-
dc.subject.MESHHeart*-
dc.subject.MESHHeart Transplantation*/pathology-
dc.subject.MESHHeart Transplantation*/physiology-
dc.subject.MESHHistidine-
dc.subject.MESHInflammation Mediators/metabolism-
dc.subject.MESHLipid Peroxidation-
dc.subject.MESHMale-
dc.subject.MESHMannitol-
dc.subject.MESHMicroscopy, Electron, Transmission-
dc.subject.MESHMyocardial Contraction-
dc.subject.MESHMyocardial Reperfusion Injury/blood-
dc.subject.MESHMyocardial Reperfusion Injury/pathology-
dc.subject.MESHMyocardial Reperfusion Injury/physiopathology-
dc.subject.MESHMyocardial Reperfusion Injury/prevention & control-
dc.subject.MESHNeutrophils/pathology-
dc.subject.MESHOrgan Preservation/methods*-
dc.subject.MESHOrgan Preservation Solutions*-
dc.subject.MESHPotassium Chloride-
dc.subject.MESHProcaine-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Inbred Lew-
dc.subject.MESHTroponin I/blood-
dc.titleSuperior myocardial preservation with HTK solution over Celsior in rat hearts with prolonged cold ischemia.-
dc.typeArticle-
dc.identifier.pmid20627336-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0039-6060(10)00204-7-
dc.contributor.affiliatedAuthor이, 성수-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.surg.2010.04.009-
dc.citation.titleSurgery-
dc.citation.volume148-
dc.citation.number2-
dc.citation.date2010-
dc.citation.startPage463-
dc.citation.endPage473-
dc.identifier.bibliographicCitationSurgery, 148(2):463-473, 2010-
dc.identifier.eissn1532-7361-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Thoracic & Cardiovascular Surgery

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