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Generation of humanized anti-DNA hydrolyzing catalytic antibodies by complementarity determining region grafting.

Authors
Kim, DS; Lee, SH; Kim, JS; Lee, SC; Kwon, MH; Kim, YS
Citation
Biochemical and biophysical research communications, 379(2):314-318, 2009
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
Humanization of nonhuman antibodies (Abs) has been carried out mainly for Abs which bind to antigen without catalytic activity. Here we report humanization of mouse-originated 3D8 (m3D8) mAbs (scFv, VH, and VL) with DNA hydrolyzing catalytic activity by grafting the complementarity determining regions (CDRs) into the corresponding regions of a fixed human framework scaffold, generating humanized 3D8 (h3D8) Abs in the respective format of scFv, VH, and VL. h3D8 Abs retained comparable DNA binding and hydrolyzing activities to those of the corresponding m3D8 Abs. Our results suggest that CDRs of anti-DNA hydrolyzing Abs might possess the intrinsic properties of DNA binding and hydrolyzing activities.
MeSH terms
Amino Acid SequenceAnimalsAntibodies, Catalytic/biosynthesis*Antibodies, Catalytic/chemistryAntibodies, Catalytic/immunologyAntibodies, Monoclonal/biosynthesis*Antibodies, Monoclonal/chemistryAntibodies, Monoclonal/immunologyComplementarity Determining Regions/biosynthesis*Complementarity Determining Regions/chemistryComplementarity Determining Regions/immunologyDNA/immunology*HumansHydrolysisMiceModels, MolecularMolecular Sequence Data
DOI
10.1016/j.bbrc.2008.12.051
PMID
19103171
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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