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Enantiomeric composition analysis of pranoprofen in equine plasma and urine by chiral liquid chromatography-tandem mass spectrometry in selected reaction monitoring mode.

Authors
Yu, J; Han, KS; Lee, G; Paik, MJ; Kim, KR
Citation
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(31):3249-3254, 2010
Journal Title
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN
1570-02321873-376X
Abstract
The enantioseparation of pranoprofen after its addition in racemic form into equine plasma and urine was conducted by chiral liquid chromatography-tandem mass spectrometry in selected reaction monitoring mode. The methods for the assay of both enantiomers were linear (r≥0.9943) in the low range from 0.001 to 0.1μg/mL and high range from 0.01 to 1.0μg/mL with good precision (% RSD≤5.6) and accuracy (% RE=-5.3 to 1.9). When racemic pranoprofen was orally administered to four horses at a single dose of 3.1mg/kg, the median plasma concentrations of (R)-pranoprofen were lower than the levels of (S)-pranoprofen from start to finish. In contrast, the urinary level of (R)-pranoprofen was 2.5 fold higher than (S)-pranoprofen level for the first 6h, followed by its rapid decrease down below (S)-pranoprofen concentration. Monitoring of the R/S ratios in equine urine may be useful for the prevention of false positive in horse doping test.
MeSH terms
Administration, OralAnimalsBenzopyrans/*blood/chemistry/pharmacokinetics/*urineChromatography, Liquid/methods/*veterinaryDoping in SportsFemaleHorses/*blood/*urineMefenamic Acid/analysis/chemistryPropionic Acids/*blood/chemistry/pharmacokinetics/*urineReproducibility of ResultsStereoisomerismTandem Mass Spectrometry/methods/*veterinary
DOI
10.1016/j.jchromb.2010.10.002
PMID
21036111
Appears in Collections:
Journal Papers > Research Organization > Institute for Neuroregeneration & Stem Cell Research
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
이, 광백, 만정
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