41 291

Cited 60 times in

Small intestine submucosa sponge for in vivo support of tissue-engineered bone formation in the presence of rat bone marrow stem cells.

Authors
Kim, KS; Lee, JY; Kang, YM; Kim, ES; Kim, GH; Rhee, SD; Cheon, HG; Kim, JH; Min, BH; Lee, HB; Kim, MS
Citation
Biomaterials, 31(6):1104-1113, 2010
Journal Title
Biomaterials
ISSN
0142-96121878-5905
Abstract
The aim of the current study was to visualize new bone formed in vivo on a small intestine submucosa (SIS) sponge used as a tissue-engineered scaffold for the repair of damaged bone. The SIS sponge provided a three-dimensional pore structure, and supported good attachment and viability of rat bone marrow stem cells (rBMSCs). To examine bone regeneration, we prepared full-thickness bilateral bone defects in the rat crania, and then treated the defects with an implanted SIS sponge or PGA mesh without or with rBMSCs, or left the defects untreated. Bone defects were evaluated by micro-CT and histologically after 2 and 4 weeks. Micro-CT demonstrated a trend toward a decrease in bone void in both the SIS sponge and SIS sponge/rBMSCs groups compared to the control and PGA mesh groups. At 4 weeks, bone formation in defects containing SIS sponge/rBMSCs was significantly greater than in all other groups. A histological analysis after 2 and 4 weeks of implantation showed localized collagen and osteocalcin deposition on SIS sponges and SIS sponges with rBMSCs. These in vivo results indicate that the SIS sponge, implanted at bone-removal defects, facilitated bone regeneration.
MeSH terms
AnimalsFemaleGuided Tissue Regeneration/methods*Intestinal Mucosa/transplantation*Mesenchymal Stem Cell Transplantation/methods*Osteogenesis*RatsRats, Inbred F344Skull Fractures/radiographySkull Fractures/surgery*SwineTissue Engineering/methods*Treatment Outcome
DOI
10.1016/j.biomaterials.2009.10.020
PMID
19854502
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
AJOU Authors
민, 병현
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse