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cAMP induces neuronal differentiation of mesenchymal stem cells via activation of extracellular signal-regulated kinase/MAPK.

Authors
Kim, SS; Choi, JM; Kim, JW; Ham, DS; Ghil, SH; Kim, MK; Kim-Kwon, Y; Hong, SY; Ahn, SC; Kim, SU; Lee, YD; Suh-Kim, H
Citation
Neuroreport, 16(12):1357-1361, 2005
Journal Title
Neuroreport
ISSN
0959-49651473-558X
Abstract
Mesenchymal stem cells are able to trans-differentiate into nonmesodermal lineage cells. Here, we identified downstream signaling molecules required for acquisition of neuron-like traits by mesenchymal stem cells following the elevation of intracellular cAMP levels. We found that forskolin induced neuron-like morphology and expression of neuron-specific enolase and neurofilament-200 in mesenchymal stem cells. Forskolin sequentially activated protein kinase A and B-regulation of alpha-fetoprotein (Raf), which led to phosphorylation of extracellular signal-regulated kinase. Importantly, blockade of extracellular signal-regulated kinase phosphorylation with a mitogen-activated protein kinase (MAPK) kinase inhibitor abrogated the forskolin-induced morphological changes and induction of neuronal proteins. These results indicate that extracellular signal-regulated kinase/MAPK mediates both cAMP-induced early cytoskeletal rearrangement and the later induction of neuronal markers in mesenchymal stem cells.
MeSH terms
AdolescentBlotting, Western/methodsCell Differentiation/drug effects*Cells, CulturedChildCyclic AMP/pharmacology*Dose-Response Relationship, DrugEnzyme Activation/drug effectsEnzyme Inhibitors/pharmacologyExtracellular Signal-Regulated MAP Kinases/metabolism*Flavonoids/pharmacologyForskolin/pharmacologyHumansMesenchymal Stem Cells/cytologyMesenchymal Stem Cells/drug effects*Phosphorylation/drug effectsTime Factorsraf Kinases/metabolism
PMID
16056139
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 성수김, 승업이, 영돈서, 해영
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