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Arachidonic acid induces neuronal death through lipoxygenase and cytochrome P450 rather than cyclooxygenase.

Authors
Kwon, KJ; Jung, YS; Lee, SH; Moon, CH; Baik, EJ
Citation
Journal of neuroscience research, 81(1):73-84, 2005
Journal Title
Journal of neuroscience research
ISSN
0360-40121097-4547
Abstract
Arachidonic acid (AA) is released from membrane phospholipids during normal and pathologic processes such as neurodegeneration. AA is metabolized via lipoxygenase (LOX)-, cyclooxygenase (COX)-, and cytochrome P450 (CYP450)-catalyzed pathways. We investigated the relative contributions of these pathways in AA-induced neuronal death. Exposure of cultured cortical neurons to AA (50 microM) yielded significantly apoptotic neuronal death, which was attenuated greatly by LOX inhibitors (nordihydroguaiaretic acid, AA861, and baicalein), or CYP450 inhibitors (SKF525A and metyrapone), rather than COX inhibitors (indomethacin and NS398). AA (10 microM)-induced neurotoxicity was prevented by all kinds of inhibitors. Compared, the neurotoxic effects of three pathway metabolites, 12-hydroxyeicosatetraenoic acid (12-HETE), a major LOX metabolite, induced a significant neurotoxicity. AA also produced reactive oxygen species within 30 min, which was reduced by all inhibitors tested, including COX inhibitors, and AA neurotoxicity was abolished by the antioxidant Trolox. AA treatment also depleted glutathione levels; this depletion was reduced by the LOX or CYP450 inhibitors rather than by the COX inhibitors. Taken together, our data suggested that the LOX pathway likely plays a major role in AA-induced neuronal death with the modification of intracellular free radical levels.
MeSH terms
Analysis of VarianceAnimalsApoptosis/physiology*Arachidonic Acid/administration & dosageArachidonic Acid/metabolism*Cerebral Cortex/cytologyCerebral Cortex/enzymologyCoculture TechniquesCytochrome P-450 Enzyme System/metabolism*Dose-Response Relationship, DrugLipoxygenase/metabolism*MiceNeuroglia/physiologyNeurons/enzymology*Prostaglandin-Endoperoxide Synthases/metabolismReactive Oxygen Species/metabolismSignal Transduction/physiologyStatistics, Nonparametric
DOI
10.1002/jnr.20520
PMID
15931672
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
권, 경자정, 이숙이, 수환문, 창현백, 은주
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