36 302

Cited 5 times in

Characterization of new microglia-like cells obtained from neonatal rat brain.

Authors
Kim, OS; Lee, CS; Kim, HY; Joe, EH; Jou, I
Citation
Biochemical and biophysical research communications, 328(1):281-287, 2005
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
We isolated spontaneously proliferating cells from primary astrocyte-enriched cultures prepared from neonatal rat brain. These cells proliferated and retained their characteristics for up to 50 generations. They expressed the microglial marker, OX42, but not glial fibrillary acidic protein, an astroglial marker. In addition, they possessed phagocytotic activity, and, when stimulated by lipopolysaccharide (LPS) or interferon-gamma (IFN-gamma), they expressed proinflammatory mediators, including cytokines (i.e., interleukin (IL)-1beta and tumor necrosis factor-alpha) and chemokines (i.e., IL-8 and monocyte chemotactic protein-1). Protein expression of inducible nitric oxide synthase and cyclooxygenase-2, and production of NO by these cells were induced by LPS or IFN-gamma. Thus, these cells possess the characteristics of microglia and can be used as a rat microglial cell line.
MeSH terms
AnimalsAnimals, NewbornCell Line/cytologyCell Line/physiologyCell ProliferationCell SizeCerebral Cortex/cytology*Cerebral Cortex/physiology*Microglia/classificationMicroglia/cytology*Microglia/physiology*RatsRats, Sprague-Dawley
DOI
10.1016/j.bbrc.2004.12.169
PMID
15670781
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Journal Papers > Research Organization > Chronic Inflammatory Disease Research Center
AJOU Authors
김, 희영조, 은혜주, 일로
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse