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Characterization of new microglia-like cells obtained from neonatal rat brain.

Authors
Kim, OS | Lee, CS | Kim, HY  | Joe, EH  | Jou, I
Citation
Biochemical and biophysical research communications, 328(1). : 281-287, 2005
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
We isolated spontaneously proliferating cells from primary astrocyte-enriched cultures prepared from neonatal rat brain. These cells proliferated and retained their characteristics for up to 50 generations. They expressed the microglial marker, OX42, but not glial fibrillary acidic protein, an astroglial marker. In addition, they possessed phagocytotic activity, and, when stimulated by lipopolysaccharide (LPS) or interferon-gamma (IFN-gamma), they expressed proinflammatory mediators, including cytokines (i.e., interleukin (IL)-1beta and tumor necrosis factor-alpha) and chemokines (i.e., IL-8 and monocyte chemotactic protein-1). Protein expression of inducible nitric oxide synthase and cyclooxygenase-2, and production of NO by these cells were induced by LPS or IFN-gamma. Thus, these cells possess the characteristics of microglia and can be used as a rat microglial cell line.
MeSH

DOI
10.1016/j.bbrc.2004.12.169
PMID
15670781
Appears in Collections:
Journal Papers > Research Organization > Inflamm-aging Translational Research Center
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Ajou Authors
김, 희영  |  조, 은혜  |  주, 일로
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