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Homomeric and heteromeric interactions of the extracellular domains of death receptors and death decoy receptors.

Authors
Lee, HW; Lee, SH; Ryu, YW; Kwon, MH; Kim, YS
Citation
Biochemical and biophysical research communications, 330(4):1205-1212, 2005
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
Death receptors (DRs) can induce apoptosis by oligomerization with TRAIL, whereas death decoy receptors (DcRs) cannot, due to their lack of functional intracellular death domains. However, it is not known whether DRs and DcRs can interact with one another to form oligomeric complexes prior to TRAIL binding. To address this issue, the extracellular domains (ECDs) of DR4 (sDR4), DR5 (sDR5), DcR1 (sDcR1), and DcR2 (sDcR2) were expressed in a soluble, monomeric form, and their binding interactions were quantified by surface plasmon resonance. The purified sDRs and sDcRs exhibited native-like secondary structure and bound to TRAIL with binding affinities in the nanomolar range (K(D)= approximately 10-62 nM), suggesting that they were properly folded and functional. The soluble receptors interacted homophilically and heterophilically with similar micromolar range affinities (K(D)= approximately 1-9 microM), with the exception that sDR5 did not interact with the sDcRs. Our results suggest that most DRs and DcRs can laterally interact through their ECDs to form homomeric and/or heteromeric complexes in the absence of TRAIL binding.
MeSH terms
Apoptosis Regulatory ProteinsDimerizationExtracellular Fluid/chemistryHumansJurkat CellsMembrane Glycoproteins/chemistry*Membrane Glycoproteins/metabolismProtein BindingProtein FoldingProtein Structure, SecondaryReceptors, Tumor Necrosis Factor/chemistry*Receptors, Tumor Necrosis Factor/metabolismSurface Plasmon ResonanceTNF-Related Apoptosis-Inducing LigandTumor Necrosis Factor-alpha/chemistry*Tumor Necrosis Factor-alpha/metabolism
DOI
10.1016/j.bbrc.2005.03.101
PMID
15823571
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
권, 명희
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