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Role of the Nfa1 protein in pathogenic Naegleria fowleri cocultured with CHO target cells.

Authors
Kang, SY; Song, KJ; Jeong, SR; Kim, JH; Park, S; Kim, K; Kwon, MH; Shin, HJ
Citation
Clinical and diagnostic laboratory immunology, 12(7):873-876, 2005
Journal Title
Clinical and diagnostic laboratory immunology
ISSN
1071-412X1098-6588
Abstract
Naegleria fowleri, a free-living amoeba, exists as a virulent pathogen which causes fatal primary amoebic meningoencephalitis in experimental animals and humans. Using infected and immune mouse sera, we previously cloned an nfa1 gene from a cDNA library of N. fowleri by immunoscreening. The nfa1 gene (360 bp) produced a recombinant 13.1-kDa protein, and the Nfa1 protein showed pseudopodium-specific immunolocalization on a trophozoite of N. fowleri. In this study, the role of the Nfa1 protein as a cell contact mechanism of N. fowleri cocultured with target cells was observed by an immunofluorescence assay with an anti-Nfa1 polyclonal antibody. Using confocal microscopic findings, the Nfa1 protein was located on the pseudopodia of N. fowleri trophozoites. The Nfa1 protein in N. fowleri trophozoites cocultured with CHO target cells was also located on pseudopodia, as well as in a food cup formed as a phagocytic structure in close contact with target cells. The amount of nfa1 mRNA of N. fowleri was strongly increased 6 h after coculture.
MeSH terms
AnimalsAntibodies, Protozoan/pharmacologyAntigens, Protozoan/biosynthesis*Antigens, Protozoan/geneticsAntigens, Protozoan/immunologyCHO CellsCricetinaeEncephalitis/immunologyEncephalitis/parasitologyMiceNaegleria fowleri/cytologyNaegleria fowleri/growth & development*Naegleria fowleri/pathogenicityPhagocytosis/drug effectsPhagocytosis/physiology*Protozoan Proteins/biosynthesis*Protozoan Proteins/geneticsProtozoan Proteins/immunologyPseudopodia/metabolism*RNA, Messenger/biosynthesisRNA, Messenger/genetics
DOI
10.1128/CDLI.12.7.873-876.2005
PMID
16002638
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
송, 경주정, 석률박, 선김, 경민권, 명희신, 호준
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