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Cytopathic changes and pro-inflammatory cytokines induced by Naegleria fowleri trophozoites in rat microglial cells and protective effects of an anti-Nfa1 antibody

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dc.contributor.authorOh, YH-
dc.contributor.authorJeong, SR-
dc.contributor.authorKim, JH-
dc.contributor.authorSong, KJ-
dc.contributor.authorKim, K-
dc.contributor.authorPark, S-
dc.contributor.authorSohn, S-
dc.contributor.authorShin, HJ-
dc.date.accessioned2011-06-16T04:50:15Z-
dc.date.available2011-06-16T04:50:15Z-
dc.date.issued2005-
dc.identifier.issn0141-9838-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/2948-
dc.description.abstractNaegleria fowleri, a free-living amoeba, causes fatal primary amoebic meningoencephalitis in experimental animals and humans. The nfa1 gene (360 bp) was previously cloned from a cDNA library of pathogenic N. fowleri by immunoscreening, and produced a 13.1-kDa recombinant protein that showed pseudopodia-specific localization by immunocytochemistry. On the basis of an idea that the pseudopodia-specific Nfa1 protein seems to be involved in the pathogenicity of N. fowleri, the cytopathic activity of N. fowleri trophozoites co-cultured with rat microglial cells was observed, and the effects of an anti-Nfa1 antibody in a co-culture system were elucidated. Using light, scanning and transmission electron microscopy, it was seen that N. fowleri trophozoites in contact with microglial cells produced vigorous pseudopodia and a food-cup structure. Microglial cells were destroyed by N. fowleri trophozoites as seen from necrotic cell death in a time-dependent manner. In a(51)Cr release assay, N. fowleri showed 17.8%, 24.9%, 54.6% and 98% cytotoxicity against microglial cells at 3, 6, 12 and 24 h post-incubation, respectively. However, when anti-Nfa1 antibody was added in a coculture system, N. fowleri cytotoxicity was reduced to 15.5%, 20.3%, 46.7% and 66.9%, respectively. Moreover, microglial cells co-cultured with N. fowleri trophozoites secreted the pro-inflammatory cytokines, TNF-alpha, IL-1beta and IL-6. In the presence of anti-Nfa1 antibody, the secretion of TNF-alpha was slightly, but not significantly, decreased.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Protozoan-
dc.subject.MESHAntigens, Protozoan-
dc.subject.MESHCell Death-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCytokines-
dc.subject.MESHFemale-
dc.subject.MESHInterleukin-1-
dc.subject.MESHInterleukin-6-
dc.subject.MESHMicroglia-
dc.subject.MESHMicroscopy, Electron-
dc.subject.MESHNaegleria fowleri-
dc.subject.MESHProtozoan Proteins-
dc.subject.MESHRats-
dc.subject.MESHTumor Necrosis Factor-alpha-
dc.titleCytopathic changes and pro-inflammatory cytokines induced by Naegleria fowleri trophozoites in rat microglial cells and protective effects of an anti-Nfa1 antibody-
dc.typeArticle-
dc.identifier.pmid16255744-
dc.identifier.urlhttp://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0141-9838&date=2005&volume=27&issue=12&spage=453-
dc.contributor.affiliatedAuthor정, 석률-
dc.contributor.affiliatedAuthor송, 경주-
dc.contributor.affiliatedAuthor김, 경민-
dc.contributor.affiliatedAuthor박, 선-
dc.contributor.affiliatedAuthor손, 성향-
dc.contributor.affiliatedAuthor신, 호준-
dc.type.localJournal Papers-
dc.identifier.doi10.1111/j.1365-3024.2005.00799.x-
dc.citation.titleParasite immunology-
dc.citation.volume27-
dc.citation.number12-
dc.citation.date2005-
dc.citation.startPage453-
dc.citation.endPage459-
dc.identifier.bibliographicCitationParasite immunology, 27(12). : 453-459, 2005-
dc.identifier.eissn1365-3024-
dc.relation.journalidJ001419838-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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