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Expression of matrix metalloproteinase-9 in pleural effusions of tuberculosis and lung cancer.

Park, KJ; Hwang, SC; Sheen, SS; Oh, YJ; Han, JH; Lee, KB
Respiration: international review of thoracic diseases, 72(2):166-175, 2005
Journal Title
Respiration: international review of thoracic diseases
BACKGROUND: Matrix metalloproteinase (MMP)-9 has been implicated in the development of pleural effusions.

OBJECTIVES: The aim of this study was to assess the expression of MMP-9 in pleural effusions of tuberculosis, lung cancer and transudates.

METHODS: Ninety-one patients (37 tuberculous pleural effusions, 42 malignant pleural effusions of lung cancer and 12 transudates) were included. Concentrations of pleural fluid MMP-9 and tissue inhibitors of matrix metalloproteinase (TIMP)-1 were determined by immunoassay. We also investigated the cellular localization of MMP-9 and TIMP-1 by reverse-transcriptase polymerase chain reaction on lymphocytes from pleural effusions and by immunohistochemical analysis of pleural tissues.

RESULTS: Pleural fluid MMP-9 levels, MMP-9/total protein and MMP-9/TIMP-1 ratios were significantly higher in tuberculous pleural effusions, whilst TIMP-1 levels were similar in the three groups. MMP-9 levels positively correlated with TIMP-1 and lactate dehydrogenase levels, and negatively with pH and glucose levels in pleural effusions. MMP-9 mRNA expression in lymphocytes tended to be higher in malignant pleural effusions of lung cancer than in the other groups without reaching statistical significance. The strongest immunoreactivity for MMP-9 was observed in epithelioid cells of tuberculous pleural tissues. Much lower levels of MMP-9 expression were found in tumor cells of pleural tissues.

CONCLUSIONS: MMP-9 is increased in tuberculous pleural effusions compared with transudates and malignant pleural effusions of lung cancer and is produced predominantly by epithelioid cells in the granulomas of tuberculous pleural tissues.
MeSH terms
AdultBiopsyCarcinoma/enzymologyCarcinoma/pathologyDiagnosis, DifferentialElectrophoresis, Agar GelExtracellular Fluid/cytologyExtracellular Fluid/enzymology*FemaleGene Expression/physiology*Gene Expression Regulation, Neoplastic/physiology*Genetic MarkersHumansImmunoenzyme TechniquesImmunohistochemistryLung Neoplasms/enzymology*Lung Neoplasms/pathologyMaleMatrix Metalloproteinase 9/genetics*Matrix Metalloproteinase 9/metabolismPleura/enzymologyPleura/pathologyPleural Effusion/enzymology*Pleural Effusion/etiologyPleural Effusion/pathologyPleural Effusion, Malignant/enzymologyPleural Effusion, Malignant/pathologyRNA/geneticsRetrospective StudiesReverse Transcriptase Polymerase Chain ReactionTissue Inhibitor of Metalloproteinase-1/geneticsTissue Inhibitor of Metalloproteinase-1/metabolismTuberculosis, Pulmonary/complicationsTuberculosis, Pulmonary/enzymology*Tuberculosis, Pulmonary/pathology
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pulmonary & Critical Care Medicine
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Science
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
AJOU Authors
박, 광주황, 성철신, 승수오, 윤정한, 재호이, 기범
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