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Neurogenin1 is sufficient to induce neuronal differentiation of embryonal carcinoma P19 cells in the absence of retinoic acid.

Kim, S; Yoon, YS; Kim, JW; Jung, M; Kim, SU; Lee, YD; Suh-Kim, H
Cellular and molecular neurobiology, 24(3):343-356, 2004
Journal Title
Cellular and molecular neurobiology
1. Neurogenin1 (Ngn1) is a basic helix-loop-helix (bHLH) transcription factor that is expressed in neuronal precursors during development of the nervous system. 2. In the present work, we investigated a instructive potential of Ngn1 in pluripotent embryonal carcinoma P19 cells. Treatment with retinoic acid (RA) induced expression of Ngn1 as well as NeuroD in P19 cells in early period of neuronal differentiation. P19 cells contained endogenous E47, a heterodimeric partner of neurogenic bHLH factors, and overexpression of Ngn1 alone was sufficient to induce the maximum activation of the E-box-mediated gene expression. 3. Sustained expression of Ngn1 in the absence of RA was sufficient to induce substantial expression of neuronal markers. The data indicate that Ngn1 is able to commit pluripotent P19 cells to adopt a neural cell phenotype in the absence of RA, which may finally lead to enhanced neuronal differentiation. The results also suggest that RA may induce neuronal differentiation of P19 cells by promoting a bHLH cascade including Ngn1.
MeSH terms
AnimalsBasic Helix-Loop-Helix Transcription FactorsBiological MarkersCOS CellsCell Differentiation/drug effects/*physiologyCell Lineage/drug effects/physiologyDNA-Binding Proteins/drug effects/metabolismEmbryonal Carcinoma Stem CellsGene Expression Regulation, Developmental/drug effects/geneticsHela CellsHelix-Loop-Helix Motifs/geneticsHumansMiceNeoplastic Stem CellsNerve Tissue Proteins/drug effects/genetics/metabolism/pharmacology/*physiologyNeurons/cytology/drug effects/*metabolismPhenotypePluripotent Stem Cells/cytology/drug effects/*metabolismSignal Transduction/drug effects/geneticsTCF Transcription FactorsTranscription Factor 7-Like 1 ProteinTranscription Factors/drug effects/genetics/metabolism/pharmacology/*physiologyTretinoin/*metabolism/pharmacology
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Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업이, 영돈서, 해영
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