34 252

Cited 34 times in

Role for PKC-epsilon in neuronal death induced by oxidative stress.

Jung, YS; Ryu, BR; Lee, BK; Mook-Jung, I; Kim, SU; Lee, SH; Baik, EJ; Moon, CH
Biochemical and biophysical research communications, 320(3):789-794, 2004
Journal Title
Biochemical and biophysical research communications
We investigated which isoforms of PKCs can be modulated and what their roles are during l-buthionine-S,R-sulfoximine (BSO)-induced neuronal death. We observed the isoform specific translocation of PKC-epsilon from the soluble fraction to the particulate in cortical neurons treated with 10 mM BSO. The translocation of PKC-epsilon by BSO was blocked by antioxidant trolox, suggesting the PKC-epsilon as a downstream of reactive oxygen species (ROS) elevated by BSO. Trolox inhibited the ROS elevation and the neuronal death in BSO-treated cortical cells. The BSO-induced neuronal death was remarkably inhibited by both the pharmacological inhibition of PKC-epsilon with epsilonV1-2 and the functional blockade for PKC-epsilon through overexpression of PKC-epsilon V1 region, suggesting the detrimental role of PKC-epsilon. These results suggest that PKC-epsilon is the major PKC isoform involved in the pathways triggered by ROS, leading to neuronal death in BSO-treated cortical neurons.
MeSH terms
AnimalsApoptosis/physiologyButhionine Sulfoximine/*pharmacologyCells, CulturedDose-Response Relationship, DrugEnzyme Activation/drug effectsGlutathione/*metabolismIsoenzymes/chemistry/metabolismMiceNeocortex/cytology/drug effects/embryology/*metabolismNeurons/cytology/drug effects/*physiologyOxidative Stress/*physiologyPeptide Fragments/pharmacologyProtein Kinase C/antagonists & inhibitors/chemistry/*metabolism/pharmacologyProtein Kinase C-epsilonReactive Oxygen Species/*metabolismStructure-Activity Relationship
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
정, 이숙김, 승업이, 수환백, 은주문, 창현
Full Text Link
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.