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Three novel cis-acting elements required for efficient plus-strand DNA synthesis of the hepatitis B virus genome.

Authors
Lee, J; Shin, MK; Lee, HJ; Yoon, G; Ryu, WS
Citation
Journal of virology, 78(14):7455-7464, 2004
Journal Title
Journal of virology
ISSN
0022-538X1098-5514
Abstract
Synthesis of the relaxed-circular (RC) DNA genomes of hepadnaviruses by reverse transcriptase involves two template switches during plus-strand DNA synthesis. These template switches require repeat sequences (so-called donor and acceptor sites) between which a complementary strand of nucleic acid is transferred. To determine cis-acting elements apart from the donor and acceptor sites that are required for plus-strand RC DNA synthesis by hepatitis B virus (HBV), a series of mutants bearing a small deletion were made and analyzed for their impact on the viral genome synthesis. We found three novel cis-acting elements in the HBV genome: one element, located in the middle of the minus strand, is indispensable, whereas the other two elements, located near either end of the minus strand, contribute modestly to the plus-strand RC DNA synthesis. The data indicated that the first element facilitates plus-strand RNA primer translocation or subsequent elongation during plus-strand RC DNA synthesis, while the last two elements, although distantly located on the minus strand, act at multiple steps to promote plus-strand RC DNA synthesis. The necessity of multiple cis-acting elements on the minus-strand template reflects the complex nature of hepadnavirus reverse transcription.
MeSH terms
Cell LineDNA, Circular/*biosynthesis/geneticsDNA, Viral/*biosynthesis/genetics*Enhancer Elements, GeneticGenome, ViralHepatitis B virus/*genetics/metabolism/physiologyHumansMutation
DOI
10.1128/JVI.78.14.7455-7464.2004
PMID
15220419
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
윤, 계순
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