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Changes in the antigenic and immunoglobulin E-binding properties of hen's egg albumin with the combination of heat and gamma irradiation treatment.

Kim, MJ; Lee, JW; Yook, HS; Lee, SY; Kim, MC; Byun, MW
Radiation physics and chemistry (Oxford, England : 1993), 72(5):645-650, 2005
Journal Title
Radiation physics and chemistry (Oxford, England : 1993)
This study was carried out to evaluate the changes in the allergenic and antigenic properties of hen's egg albumin (ovalbumin [OVA]) with the combination of heat and gamma irradiation treatment. OVA solution samples were treated by (i) heating (sample 1), (ii) irradiation after heating (sample 2), and (iii) heating after irradiation (sample 3). Samples were isothermally heated and irradiated at the absorption dose of 10 kGy. Competitive indirect enzyme-linked immunosorbent assays (ELISAs) were performed with blood serum to test the ability of treated OVA to bind to immunoglobulin E (IgE) and mouse murine monoclonal antibody (IgG). OVA's ability to bind to mouse IgG changed upon heating at 75 degrees C, and its ability to bind to egg-allergic IgE changed upon heating at 80 degrees C. The ELISAs showed that egg-allergic IgE did not recognize OVA very well when heated at > or = 80 degrees C, while mouse IgG retained better activity under these conditions. Egg-allergic IgE binding was low both for OVA samples treated by heating and for samples treated by irradiation followed by heating. These results show that allergies induced by OVA could be effectively reduced by the combination of heat and gamma irradiation treatment.
MeSH terms
AnimalsBinding, CompetitiveEgg Proteins/immunology*Egg Proteins/radiation effectsEnzyme-Linked Immunosorbent AssayFood Hypersensitivity/prevention & control*Gamma RaysHot TemperatureHumansImmunoglobulin E/immunology*Immunoglobulin G/immunology*MiceOvalbumin/immunology*Ovalbumin/radiation effectsProtein Denaturation
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Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
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