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Murine model of buckwheat allergy by intragastric sensitization with fresh buckwheat flour extract.

Authors
Lee, SY; Oh, S; Lee, K; Jang, YJ; Sohn, MH; Lee, KE; Kim, KE
Citation
Journal of Korean medical science, 20(4):566-572, 2005
Journal Title
Journal of Korean medical science
ISSN
1011-89341598-6357
Abstract
Food allergies affect about 4% of the Korean population, and buckwheat allergy is one of the most severe food allergies in Korea. The purpose of the present study was to develop a murine model of IgE-mediated buckwheat hypersensitivity induced by intragastric sensitization. Young female C3H/HeJ mice were sensitized and challenged intragastricly with fresh buckwheat flour (1, 5, 25 mg/dose of proteins) mixed in cholera toxin, followed by intragastric challenge. Anaphylactic reactions, antigen-specific antibodies, splenocytes proliferation assays and cytokine productions were evaluated. Oral buckwheat challenges of sensitized mice provoked anaphylactic reactions such as severe scratch, perioral/periorbital swellings, or decreased activity. Reactions were associated with elevated levels of buckwheatspecific IgE antibodies. Splenocytes from buckwheat allergic mice exhibited significantly greater proliferative responses to buckwheat than non-allergic mice. Buckwheat-stimulated IL-4, IL-5, and INF-gamma productions were associated with elevated levels of buckwheat-specific IgE in sensitized mice. In this model, 1 mg and 5 mg dose of sensitization produced almost the same degree of Th2-directed immune response, however, a 25 mg dose showed blunted antibody responses. In conclusion, we developed IgE-mediated buckwheat allergy by intragastric sensitization and challenge, and this model could provide a good tool for future studies.
MeSH terms
Anaphylaxis/bloodAnaphylaxis/immunologyAnimalsCell Proliferation/drug effectsDisease Models, AnimalDose-Response Relationship, DrugEnzyme-Linked Immunosorbent AssayFagopyrum/immunology*FemaleFlour*Food Hypersensitivity/bloodFood Hypersensitivity/immunology*Immunoglobulin E/bloodImmunoglobulin E/immunologyImmunoglobulin G/bloodImmunoglobulin G/immunologyInterferon-gamma/biosynthesisInterleukin-4/biosynthesisInterleukin-5/biosynthesisMiceMice, Inbred C3HPlant Extracts/administration & dosagePlant Extracts/immunologySpleen/cytologySpleen/drug effectsSpleen/metabolismStomach/drug effectsStomach/immunology*T-Lymphocytes/cytologyT-Lymphocytes/drug effectsT-Lymphocytes/metabolismTime Factors
DOI
10.3346/jkms.2005.20.4.566
PMID
16100445
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
이, 수영장, 영주
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