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Oligomer synthesis by priming deficient polymerase in hepatitis B virus core particle.

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dc.contributor.authorKim, HY-
dc.contributor.authorPark, GS-
dc.contributor.authorKim, EG-
dc.contributor.authorKang, SH-
dc.contributor.authorShin, HJ-
dc.contributor.authorPark, S-
dc.contributor.authorKim, KH-
dc.date.accessioned2011-06-28T02:37:04Z-
dc.date.available2011-06-28T02:37:04Z-
dc.date.issued2004-
dc.identifier.issn0042-6822-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3069-
dc.description.abstractHepadnavirus DNA polymerase functions in DNA synthesis and encapsidation, and acts as a primer for minus-strand DNA synthesis. Through protein priming reaction, a short DNA oligomer synthesized from the bulge of epsilon as template is covalently attached to the Tyr residue in the terminal protein (TP) domain of DNA polymerase. Using endogenous polymerase assays and native agarose gel analysis, we detected endogenous polymerase activity in priming-deficient mutant core particles, but not in reverse transcriptase (RT) reaction- or P protein-deficient mutant core particles. In addition, priming-deficient mutant core particles incorporated radiolabeled (32)P-dATP, (32)P-TTP, and (32)P-dGTP, but not (32)P-dCTP. Our results suggest that the priming-deficient mutant P protein has the ability to synthesize oligomers (presumably nascent minus-strand DNA) in the absence of covalent linkage between TP and the first deoxynucleotide. We propose that the priming-deficient mutant may be defective in minus-strand DNA translocation to direct repeat (DR) 1 at the 3' end of pregenomic RNA (pgRNA) that leads to the elongation of minus-strand DNA.-
dc.language.isoen-
dc.subject.MESHDNA, Single-Stranded-
dc.subject.MESHDNA, Viral-
dc.subject.MESHGene Products, pol-
dc.subject.MESHHepatitis B virus-
dc.subject.MESHOligopeptides-
dc.subject.MESHRNA-Directed DNA Polymerase-
dc.subject.MESHRepetitive Sequences, Nucleic Acid-
dc.subject.MESHVirus Replication-
dc.titleOligomer synthesis by priming deficient polymerase in hepatitis B virus core particle.-
dc.typeArticle-
dc.identifier.pmid15063113-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0042682204000315-
dc.contributor.affiliatedAuthor신, 호준-
dc.contributor.affiliatedAuthor박, 선-
dc.contributor.affiliatedAuthor김, 경민-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.virol.2004.01.009-
dc.citation.titleVirology-
dc.citation.volume322-
dc.citation.number1-
dc.citation.date2004-
dc.citation.startPage22-
dc.citation.endPage30-
dc.identifier.bibliographicCitationVirology, 322(1). : 22-30, 2004-
dc.identifier.eissn1096-0341-
dc.relation.journalidJ000426822-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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