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Mesenchymal stem cells from cryopreserved human umbilical cord blood.

Authors
Lee, MW; Choi, J; Yang, MS; Moon, YJ; Park, JS; Kim, HC; Kim, YJ
Citation
Biochemical and biophysical research communications, 320(1):273-278, 2004
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
Umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages, but the presence of mesenchymal stem cells (MSCs) in UCB has been disputed and it remains to be validated. In this study, we examined the ability of cryopreserved UCB harvests to produce cells with characteristics of MSCs. We were able to obtain homogeneous plastic adherent cells from the mononuclear cell fractions of cryopreserved UCB using our culture conditions. These adherent cell populations exhibited fibroblast-like morphology and typical mesenchymal-like immunophenotypes (CD73+, CD105+, and CD166+, etc.). These cells presented the self-renewal capacity and the mesenchymal cell-lineage potential to form bone, fat, and cartilage. Moreover, they expressed mRNAs of multi-lineage genes including SDF-1, NeuroD, and VEGF-R1, suggesting that the obtained cells had the multi-differentiation capacity as bone marrow-derived MSCs. These results indicate that cryopreserved human UCB fractions can be used as an alternative source of MSCs for experimental and therapeutic applications.
MeSH terms
Antigens, Surface/*metabolismBone Marrow Cells/cytology/metabolismCell Division/physiologyCell Separation/*methodsCells, CulturedCryopreservation/*methods*Fetal BloodHematopoietic Stem Cell Mobilization/*methodsHumansMesenchymal Stem Cells/*cytology/*metabolism
DOI
10.1016/j.bbrc.2004.04.206
PMID
15207732
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
AJOU Authors
박, 준성김, 효철
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