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Simultaneous establishment of pancreatic cancer organoid and cancer-associated fibroblast using a single-pass endoscopic ultrasound-guided fine-needle biopsy specimen

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dc.contributor.authorKim, S-
dc.contributor.authorWoo, KJ-
dc.contributor.authorYang, CM-
dc.contributor.authorPark, SH-
dc.contributor.authorHwang, JC-
dc.contributor.authorYoo, BM-
dc.contributor.authorKim, JH-
dc.contributor.authorLee, D-
dc.contributor.authorYang, MJ-
dc.date.accessioned2023-12-11T05:42:36Z-
dc.date.available2023-12-11T05:42:36Z-
dc.date.issued2023-
dc.identifier.issn0915-5635-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/32016-
dc.description.abstractConsidering the critical roles of cancer-associated fibroblasts (CAFs) in pancreatic cancer, recent studies have attempted to incorporate stromal elements into organoid models to recapitulate the tumor microenvironment. This study aimed to evaluate the feasibility of patient-derived organoid (PDO) and CAF cultures by using single-pass endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) samples from prospectively enrolled pancreatic cancer patients. The obtained samples were split into two portions for PDO and CAF cultures. PDOs and CAFs were cultured successfully in 54.4% (31/57) and 47.4% (27/57) of the cases, respectively. Both components were established in 21 cases (36.8%). Various clinicopathologic factors, including the tumor size, tumor location, clinical stage, histologic subtype, and tumor differentiation, did not influence the PDO establishment. Instead, the presence of necrosis in tumor samples was associated with initial PDO generation but no further propagation beyond passage 5 (P = 0.024). The “poorly cohesive cell carcinoma pattern” also negatively influenced the PDO establishment (P = 0.018). Higher stromal proportion in tumor samples was a decisive factor for successful CAF culture (P = 0.005). Our study demonstrated that the coestablishment of PDOs and CAFs is feasible even with a single-pass EUS-FNB sample, implying an expanding role of endoscopists in future precision medicine.-
dc.language.isoen-
dc.subject.MESHCancer-Associated Fibroblasts-
dc.subject.MESHEndoscopic Ultrasound-Guided Fine Needle Aspiration-
dc.subject.MESHHumans-
dc.subject.MESHOrganoids-
dc.subject.MESHPancreatic Carcinoma-
dc.subject.MESHPancreatic Neoplasms-
dc.subject.MESHTumor Microenvironment-
dc.titleSimultaneous establishment of pancreatic cancer organoid and cancer-associated fibroblast using a single-pass endoscopic ultrasound-guided fine-needle biopsy specimen-
dc.typeArticle-
dc.identifier.pmid37522250-
dc.subject.keywordcancer-associated fibroblast-
dc.subject.keywordendoscopic ultrasound-guided fine-needle biopsy-
dc.subject.keywordpancreatic cancer-
dc.subject.keywordpatient-derived organoid-
dc.contributor.affiliatedAuthorKim, S-
dc.contributor.affiliatedAuthorHwang, JC-
dc.contributor.affiliatedAuthorYoo, BM-
dc.contributor.affiliatedAuthorLee, D-
dc.contributor.affiliatedAuthorYang, MJ-
dc.type.localJournal Papers-
dc.identifier.doi10.1111/den.14648-
dc.citation.titleDigestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society-
dc.citation.volume35-
dc.citation.number7-
dc.citation.date2023-
dc.citation.startPage918-
dc.citation.endPage926-
dc.identifier.bibliographicCitationDigestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society, 35(7). : 918-926, 2023-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn1443-1661-
dc.relation.journalidJ009155635-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
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