3 163

Cited 106 times in

Intravenous administration of human neural stem cells induces functional recovery in Huntington's disease rat model.

Authors
Lee, ST; Chu, K; Park, JE; Lee, K; Kang, L; Kim, SU; Kim, M
Citation
Neuroscience research, 52(3):243-249, 2005
Journal Title
Neuroscience research
ISSN
0168-01021872-8111
Abstract
An animal model induced by striatal quinolinic acid (QA) injection shows ongoing striatal degeneration mimicking Huntington's disease. To study the migratory ability and the neuroprotective effect of human neural stem cells (NSCs) in this model, we transplanted NSCs (5 x 10(6)) or saline intravenously at 7 days after unilateral QA injection. NSCs-group exhibited the reduced apomorphine-induced rotation and the reduced striatal atrophy compared to the control. PCR analysis for the human-specific ERV-3 gene supported an evidence of the engraftment of human NSCs in the rat brain. X-gal+ cells were found in and around the damaged striatum and migrated NSCs differentiated into neurons and glias. This result indicates that intravenously injected human NSCs can migrate into the striatal lesion, decrease the following striatal atrophy, and induce long-term functional improvement in a glutamate toxicity-induced striatal degeneration model.
MeSH terms
AnimalsApomorphine/pharmacologyBehavior, Animal/drug effectsBehavior, Animal/physiologyCell Count/methodsCells, CulturedCorpus Striatum/drug effectsCorpus Striatum/metabolismCorpus Striatum/pathologyDNA-Binding Proteins/metabolismDisease Models, AnimalDopamine and cAMP-Regulated Phosphoprotein 32Drosophila Proteins/metabolismEndogenous Retroviruses/geneticsFunctional LateralityGalactosides/diagnostic useGlial Fibrillary Acidic Protein/metabolismHumansHuntington Disease/chemically inducedHuntington Disease/therapy*Immunohistochemistry/methodsIndoles/diagnostic useInfusions, Intravenous/methods*MaleMotor Activity/drug effectsMotor Activity/physiologyNerve Tissue Proteins/metabolismNeurons/physiology*Parvalbumins/metabolismPhosphoproteins/metabolismPhosphopyruvate Hydratase/metabolismQuinolinic AcidRNA, Messenger/biosynthesisRandom AllocationRatsRats, Sprague-DawleyRecovery of Function/physiology*Reverse Transcriptase Polymerase Chain Reaction/methodsRotarod Performance Test/methodsStem Cell Transplantation/methods*Stem Cells/physiology*Time Factorsgamma-Aminobutyric Acid/metabolism
DOI
10.1016/j.neures.2005.03.016
PMID
15896865
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse