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Exosomes Secreted During Myogenic Differentiation of Human Fetal Cartilage-Derived Progenitor Cells Promote Skeletal Muscle Regeneration through miR-145-5p

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dc.contributor.authorShin, DI-
dc.contributor.authorJin, YJ-
dc.contributor.authorNoh, S-
dc.contributor.authorYun, HW-
dc.contributor.authorPark, DY-
dc.contributor.authorMin, BH-
dc.date.accessioned2024-06-19T07:06:59Z-
dc.date.available2024-06-19T07:06:59Z-
dc.date.issued2024-
dc.identifier.issn1738-2696-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/32524-
dc.description.abstractBackground: Currently, there is no apparent treatment for sarcopenia, which is characterized by diminished myoblast function. We aimed to manufacture exosomes that retain the myogenic differentiation capacity of human fetal cartilage-derived progenitor cells (hFCPCs) and investigate their muscle regenerative efficacy in myoblasts and a sarcopenia rat model. Methods: The muscle regeneration potential of exosomes (F-Exo) secreted during myogenic differentiation of hFCPCs was compared to human bone marrow mesenchymal stem cells-derived (hBMSCs) exosomes (B-Exo) in myoblasts and sarcopenia rat model. The effect of F-Exo was analyzed through known microRNAs (miRNAs) analysis. The mechanism of action of F-Exo was confirmed by measuring the expression of proteins involved in the Wnt signaling pathway. Results: F-Exo and B-Exo showed similar exosome characteristics. However, F-Exo induced the expression of muscle markers (MyoD, MyoG, and MyHC) and myotube formation in myoblasts more effectively than B-Exo. Moreover, F-Exo induced greater increases in muscle fiber cross-sectional area and muscle mass compared to B-Exo in a sarcopenia rat. The miR-145-5p, relevant to muscle regeneration, was found in high concentrations in the F-Exo, and RNase pretreatment reduced the efficacy of exosomes. The effects of F-Exo on the expression of myogenic markers in myoblasts were paralleled by the miR-145-5p mimics, while the inhibitor partially negated this effect. F-Exo was involved in the Wnt signaling pathway by enhancing the expression of Wnt5a and β-catenin. Conclusion: F-Exo improved muscle regeneration by activating the Wnt signaling pathway via abundant miR-145-5p, mimicking the remarkable myogenic differentiation potential of hFCPCs.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHCartilage-
dc.subject.MESHExosomes-
dc.subject.MESHHumans-
dc.subject.MESHMesenchymal Stem Cells-
dc.subject.MESHMicroRNAs-
dc.subject.MESHMuscle, Skeletal-
dc.subject.MESHRats-
dc.subject.MESHSarcopenia-
dc.titleExosomes Secreted During Myogenic Differentiation of Human Fetal Cartilage-Derived Progenitor Cells Promote Skeletal Muscle Regeneration through miR-145-5p-
dc.typeArticle-
dc.identifier.pmid38294592-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10987463-
dc.subject.keywordExosomes-
dc.subject.keywordFetal cartilage-derived progenitor cells-
dc.subject.keywordmiR-145-5p-
dc.subject.keywordSarcopenia-
dc.subject.keywordWnt signaling pathway-
dc.contributor.affiliatedAuthorYun, HW-
dc.contributor.affiliatedAuthorPark, DY-
dc.type.localJournal Papers-
dc.identifier.doi10.1007/s13770-023-00618-w-
dc.citation.titleTissue engineering and regenerative medicine-
dc.citation.volume21-
dc.citation.number3-
dc.citation.date2024-
dc.citation.startPage487-
dc.citation.endPage497-
dc.identifier.bibliographicCitationTissue engineering and regenerative medicine, 21(3). : 487-497, 2024-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn2212-5469-
dc.relation.journalidJ017382696-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
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