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Perturbations in calcium-mediated signal transduction in microglia from Alzheimer's disease patients.

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dc.contributor.authorMcLarnon, JG-
dc.contributor.authorChoi, HB-
dc.contributor.authorLue, LF-
dc.contributor.authorWalker, DG-
dc.contributor.authorKim, SU-
dc.date.accessioned2011-07-08T02:23:19Z-
dc.date.available2011-07-08T02:23:19Z-
dc.date.issued2005-
dc.identifier.issn0360-4012-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3256-
dc.description.abstractCalcium-sensitive fluorescence microscopy has been used to study Ca2+-dependent signal transduction pathways in microglia obtained from Alzheimer's disease (AD) patients and non-demented (ND) individuals. Data were obtained from nine AD cases and seven ND individuals and included basal levels of intracellular Ca2+ [Ca2+]i, peak amplitudes (Delta[Ca2+]i) and time courses of adenosine triphosphate (ATP) responses and amplitudes of an initial transient response and a subsequent second component of Ca2+ influx through store-operated channels (SOC) induced by platelet-activating factor (PAF). Overall, AD microglia were characterized by significantly higher (20%) basal Ca2+ [Ca2+]i relative to ND cells. The Delta[Ca2+]i of ATP and initial phase of PAF responses, which reflect rapid depletion of Ca2+ from endoplasmic reticulum stores, were reduced by respective values of 63% and 59% in AD cells relative to amplitudes recorded from ND microglia. Additionally, AD microglia showed diminished amplitudes (reduction of 61%) of SOC-mediated Ca2+ entry induced by PAF and prolonged time courses (increase of 60%) of ATP responses with respect to ND microglia. We have generally replicated these results with exposure of human fetal microglia to beta amyloid (5 microM Abeta1-42 applied for 24 hr). Overall, these data indicate significant abnormalities are present in Ca2+-mediated signal transduction in microglia isolated from AD patients.-
dc.language.isoen-
dc.subject.MESHAdenosine Triphosphate-
dc.subject.MESHAlzheimer Disease-
dc.subject.MESHAmyloid beta-Peptides-
dc.subject.MESHAnalysis of Variance-
dc.subject.MESHCalcium-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDrug Interactions-
dc.subject.MESHFetus-
dc.subject.MESHHumans-
dc.subject.MESHMicroglia-
dc.subject.MESHPeptide Fragments-
dc.subject.MESHPlatelet Activating Factor-
dc.subject.MESHPostmortem Changes-
dc.subject.MESHSignal Transduction-
dc.subject.MESHSpectrometry, Fluorescence-
dc.titlePerturbations in calcium-mediated signal transduction in microglia from Alzheimer's disease patients.-
dc.typeArticle-
dc.identifier.pmid15948178-
dc.contributor.affiliatedAuthor김, 승업-
dc.type.localJournal Papers-
dc.identifier.doi10.1002/jnr.20487-
dc.citation.titleJournal of neuroscience research-
dc.citation.volume81-
dc.citation.number3-
dc.citation.date2005-
dc.citation.startPage426-
dc.citation.endPage435-
dc.identifier.bibliographicCitationJournal of neuroscience research, 81(3). : 426-435, 2005-
dc.identifier.eissn1097-4547-
dc.relation.journalidJ003604012-
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Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
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