5 258

Cited 39 times in

Neuronal cell death induced by cystatin C in vivo and in cultured human CNS neurons is inhibited with cathepsin B.

Authors
Nagai, A; Ryu, JK; Terashima, M; Tanigawa, Y; Wakabayashi, K; McLarnon, JG; Kobayashi, S; Masuda, J; Kim, SU
Citation
Brain research, 1066(1-2):120-128, 2005
Journal Title
Brain research
ISSN
0006-89931872-6240
Abstract
Cystatin C, a cysteine protease inhibitor, is implicated in pathogenesis of late-onset Alzheimer's disease and other neurological disorders. Our recent study showed that cystatin C injection into rat hippocampus induced neuronal cell death in granule cell layer of dentate gyrus in vivo. We further confirmed that cystatin C neurotoxicity was inhibited by simultaneous coapplication of cathepsin B, a cysteine protease. In vitro cytotoxicity was also studied in cultures of human CNS neurons, mixed cultures with astrocytes and A1 human hybrid neurons. Cystatin C induced neuronal cell death in a dose-dependent manner, which accompanied increased number of TUNEL (+) cells, up-regulation of active caspase-3 and DNA ladder. The results of the present study indicate that cystatin C participates in the process of apoptotic neuronal cell death in experimental conditions by means of inhibitory activity of cysteine proteases, and that cystatin C might be involved in the pathogenesis in human neurological disorders including Alzheimer's disease.
MeSH terms
AnimalsApoptosisAstrocytes/drug effectsCaspase 3Caspases/metabolismCathepsin B/pharmacology*Cell DeathCell SurvivalCells, CulturedCentral Nervous System/cytology*Central Nervous System/drug effectsCystatin CCystatins/antagonists & inhibitorsCystatins/pharmacology*DNA/biosynthesisDNA/geneticsHippocampusHumansHybrid CellsImmunohistochemistryIn Situ Nick-End LabelingMaleMicroinjectionsNeurons/physiology*RatsRats, Sprague-DawleyTetrazolium SaltsThiazoles
DOI
10.1016/j.brainres.2005.10.063
PMID
16325785
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse