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The dispensability of VH-VL pairing and the indispensability of VL domain integrity in the IgG1 secretion process

Authors
Choi, J | Jeon, Y | Roh, Y | Jang, J | Lee, E | Villamante, L | Kim, M | Kwon, MH
Citation
Frontiers in molecular biosciences, 11. : 1346259-1346259, 2024
Journal Title
Frontiers in molecular biosciences
ISSN
2296-889X
Abstract
Introduction: The CH1 domain of IgG antibodies controls assembly and secretion, mediated by the molecular chaperone BiP via the endoplasmic reticulum protein quality control (ERQC) mechanism. However, it is not clear whether the variable domains are necessary for this process. Methods: Here, we generated IgG1 antibodies in which the V domain (VH and/or VL) was either removed or replaced, and then assessed expression, assembly, and secretion in HEK293 cells. Results: All Ig variants formed a covalent linkage between the Cγ1 and Cκ, were successfully secreted in an assembled form. Replacement of the cognate Vκ with a non-secretory pseudo Vκ (ψVκ) hindered secretion of individual or assembled secretion of neither heavy chains (HCs) nor light chains (LCs). The ψLC (ψVκ-Cκ) exhibited a less folded structure compared to the wild type (wt) LC, as evidenced by enhanced stable binding to the molecular chaperone BiP and susceptibility to proteolytic degradation. Molecular dynamics simulation demonstrated dramatic alterations in overall structure of ψFab (Fd-ψLC) from wt Fab. Discussion: These findings suggest that V domains do not initiate HC:LC assembly and secretion; instead, the critical factor governing IgG assembly and secretion is the CH-CL pairing. Additionally, the structural integrity of the VL domain is crucial for IgG secretion. These data offer valuable insight into the design of bioactive molecules based on an IgG backbone.
Keywords

DOI
10.3389/fmolb.2024.1346259
PMID
38756530
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Ajou Authors
권, 명희
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