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Synergism of Helicobacter pylori infection and stress on the augmentation of gastric mucosal damage and its prevention with alpha-tocopherol.

Authors
Oh, TY; Yeo, M; Han, SU; Cho, YK; Kim, YB; Chung, MH; Kim, YS; Cho, SW; Hahm, KB
Citation
Free radical biology & medicine, 38(11):1447-1457, 2005
Journal Title
Free radical biology & medicine
ISSN
0891-58491873-4596
Abstract
Despite evidence that Helicobacter pylori (H. pylori) infection is closely associated with stress in gastric ulcer patients, the underlying mechanism why ulcer recurrence after stress is augmented especially in patients with H. pylori remains unknown. In this study, we found that oxidative stress played a critical role in the augmented mucosal damage provoked by water immersion restraint stress (WIRS) in H. pylori infection and that an antioxidant, alpha-tocopherol, could ameliorate the aggravation of stress-associated gastric mucosal damage. Two hundred forty SD rats were divided into two groups according to H. pylori inoculation, and after 24 weeks of H. pylori infection, the water immersion restraint stress was imposed for 30, 120, or 480 min, respectively. To evaluate the therapeutic effects of an antioxidant, alpha-tocopherol was administrated 40 mg/kg daily prior to imposing WIRS. Remarkably increased hemorrhagic lesions and bleeding indexes were noted in the H. pylori-infected group with statistical significance (P < 0.05) compared to the noninfected group at the same duration of WIRS. Significantly higher oxidative stress documented by iNOS, lipid peroxides, and GSH level was detected in gastric homogenates of the H. pylori-infected group. Proteomic analysis using 2-dimensional electrophoresis showed a decrease of HSP27 and other chaperone proteins. alpha-Tocopherol pretreatment significantly prevented the gastric mucosal damage, caused by WIRS in the presence of H. pylori. alpha-Tocopherol induced HSP27 expression, which was well correlated with downregulation of iNOS mRNA. Conclusively, the presence of H. pylori caused significant deterioration of stress-induced gastric mucosal lesions through increased oxidative stress and thus antioxidant treatment such as alpha-tocopherol protected the gastric injuries.
MeSH terms
AnimalsAntioxidants/metabolismAntioxidants/pharmacologyBlotting, WesternCells, CulturedCytokines/metabolismDisease Models, AnimalDown-RegulationElectrophoresis, Gel, Two-DimensionalEpithelial Cells/cytologyGastric Mucosa/injuriesGastric Mucosa/pathology*Glutathione/metabolismHSP27 Heat-Shock ProteinsHeat-Shock Proteins/metabolismHelicobacter Infections/complications*Helicobacter pylori/metabolismLipid PeroxidationMaleMolecular Chaperones/metabolismNeoplasm Proteins/metabolismNitric Oxide Synthase/metabolismNitric Oxide Synthase Type IIOxidative StressProteomicsRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStomach Ulcer/prevention & control*Thiobarbituric Acid Reactive SubstancesTime Factorsalpha-Tocopherol/metabolismalpha-Tocopherol/pharmacology*
DOI
10.1016/j.freeradbiomed.2005.02.005
PMID
15890619
Appears in Collections:
Journal Papers > Research Organization > Genomic Research Center for Gastroenterology
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
AJOU Authors
여, 말희한, 상욱조, 용관김, 영배조, 성원함, 기백
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