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Modulation of the N-type calcium channel gene expression by the alpha subunit of Go.

Authors
Kim, BJ; Ghil, SH; Kim, MJ; Yun Park, S; Kim, DS; Hwan Kim, S; Chin, H; Birnbaumer, L; Jiang, M; Hong, SY; Suh-Kim, H; Lee, YD
Citation
Brain research. Molecular brain research, 112(1-2):95-102, 2003
Journal Title
Brain research. Molecular brain research
ISSN
0169-328X
Abstract
Go, a heterotrimeric G-protein, is enriched in brain and neuronal growth cones. Although several reports suggest that Go may be involved in modulation of neuronal differentiation, the precise role of Go is not clear. To investigate the function of Go in neuronal differentiation, we determined the effect of Goalpha, the alpha subunit of Go, on the expression of Ca(v)2.2, the pore-forming unit of N-type calcium channels, at the transcription level. Treatment with cyclic AMP (cAMP), which triggers neurite outgrowth in neuroblastoma F11 cells, increased the mRNA level and the promoter activity of the Ca(v)2.2 gene. Overexpression of Goalpha inhibited neurite extension in F11 cells and simultaneously repressed the stimulatory effect of cAMP on the Ca(v)2.2 gene expression to the basal level. Targeted mutation of the Goalpha gene also increased the level of Ca(v)2.2 in the brain. These results suggest that Go may regulate neuronal differentiation through modulation of gene expression of target genes such as N-type calcium channels.
MeSH terms
AnimalsBrain/embryology*Brain/metabolism*Calcium Channels, N-Type/biosynthesis*Calcium Channels, N-Type/geneticsCell Differentiation/drug effectsCell Differentiation/genetics*Cell LineCyclic AMP/metabolismCyclic AMP/pharmacologyGTP-Binding Protein alpha Subunits, Gi-GoGene Expression Regulation, Developmental/genetics*Gene TargetingGrowth Cones/drug effectsGrowth Cones/metabolism*Heterotrimeric GTP-Binding Proteins/geneticsHeterotrimeric GTP-Binding Proteins/metabolism*MiceMice, KnockoutMutation/geneticsPromoter Regions, Genetic/geneticsProtein Subunits/geneticsProtein Subunits/metabolismRNA, Messenger/drug effectsRNA, Messenger/metabolismUp-Regulation/drug effectsUp-Regulation/genetics
PMID
12670707
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
AJOU Authors
김, 성환서, 해영이, 영돈
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