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Wortmannin enhances lipopolysaccharide-induced inducible nitric oxide synthase expression in microglia in the presence of astrocytes in rats.

Authors
Pyo, H; Yang, MS; Jou, I; Joe, EH
Citation
Neuroscience letters, 346(3):141-144, 2003
Journal Title
Neuroscience letters
ISSN
0304-39401872-7972
Abstract
Microglia, the primary inflammatory cells in the brain, are activated upon brain injury. Activated microglia produce nitric oxide (NO), a major toxin to neuronal cells. It has been reported that astrocytes inhibit microglial activation. In this study, we found that wortmannin, a natural inhibitor of phosphatidylinositol 3-kinase, significantly increased lipopolysaccharide (LPS)-induced NO release and inducible nitric oxide synthase (iNOS) expression in microglia in the presence but not in the absence of astrocytes. In response to LPS even in the presence of wortmannin, iNOS immunoreactivity was detected in microglia but not in astrocytes. These results suggest that astrocytes could regulate microglia-mediated brain inflammation by inhibiting microglial NO release/iNOS expression via a wortmannin-sensitive mechanism.
MeSH terms
Androstadienes/pharmacology*AnimalsAnimals, NewbornAstrocytes/cytologyAstrocytes/drug effects*Astrocytes/enzymologyImmunoblottingImmunohistochemistryLipopolysaccharides/pharmacology*Microglia/cytologyMicroglia/drug effects*Microglia/enzymologyNitric Oxide Synthase/metabolism*Nitric Oxide Synthase Type IIPhosphatidylinositol 3-Kinases/antagonists & inhibitors*RatsRats, Sprague-DawleySalmonella enteritidis
PMID
12853104
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
양, 명순주, 일로조, 은혜
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