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Interleukin-6 small interfering RNA improved the herpes simplex virus-induced systemic inflammation in vivo Behcet's disease-like mouse model.

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dc.contributor.authorShim, J-
dc.contributor.authorByun, HO-
dc.contributor.authorLee, YD-
dc.contributor.authorLee, ES-
dc.contributor.authorSohn, S-
dc.date.accessioned2010-11-29T02:33:32Z-
dc.date.available2010-11-29T02:33:32Z-
dc.date.issued2009-
dc.identifier.issn0969-7128-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/329-
dc.description.abstractIt is known that the level of interleukin-6 (IL-6) is higher in patients with active Behcet's disease (BD) than in those with inactive disease. Herpes simplex virus (HSV) type 1 inoculation of the earlobes of ICR mice resulted in the development of BD-like symptoms. To find out whether downregulation of IL-6 would affect the symptoms of BD, IL-6 small interfering RNA (siRNA) was administered to a BD mouse model. IL-6 siRNA was intraperitoneally injected into BD mice to downregulate IL-6 (n=9). IL-6 siRNA injection downregulated serum IL-6 level (118.9+/-114.4 pg ml(-1)) compared with scramble injection (439.4+/-378.0 pg ml(-1)) in BD mice (P=0.01). In seven out of nine IL-6 siRNA-injected BD mice, 77.8% improved and the severity score was decreased from 3.1+/-1.05 to 1.7+/-0.87 (P=0.005), whereas two out of six (33.3%) scramble-injected BD mice improved and the severity score changed from 2.5+/-0.84 to 2.0+/-1.41 (P=0.203). Foxp3, ROR gamma t, IL-17A, IL-17F and tumor necrosis factor-alpha were also influenced in IL-6 siRNA-injected BD mice compared with scramble-injected BD mice. Adoptive transfer of CD4+CD25+ cells to BD mice affected the decrease of IL-6 serum levels and were dependent on CD4+CD25+ cell numbers. These results showed that downregulation of IL-6 improved the inflammatory symptoms in BD mice through upregulation of regulatory T cells and inhibition of Th17 cells.-
dc.language.isoen-
dc.subject.MESHAdoptive Transfer-
dc.subject.MESHAnimals-
dc.subject.MESHBehcet Syndrome-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHDown-Regulation-
dc.subject.MESHGene Therapy-
dc.subject.MESHHerpes Simplex-
dc.subject.MESHHerpesvirus 1, Human-
dc.subject.MESHInterleukin-17-
dc.subject.MESHInterleukin-23-
dc.subject.MESHInterleukin-6-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred ICR-
dc.subject.MESHRNA Interference-
dc.subject.MESHRNA, Small Interfering-
dc.subject.MESHT-Lymphocytes, Regulatory-
dc.subject.MESHTransforming Growth Factor beta-
dc.subject.MESHTreatment Outcome-
dc.subject.MESHTumor Necrosis Factor-alpha-
dc.subject.MESHUp-Regulation-
dc.titleInterleukin-6 small interfering RNA improved the herpes simplex virus-induced systemic inflammation in vivo Behcet's disease-like mouse model.-
dc.typeArticle-
dc.identifier.pmid19092856-
dc.contributor.affiliatedAuthor이, 영돈-
dc.contributor.affiliatedAuthor이, 은소-
dc.contributor.affiliatedAuthor손, 성향-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/gt.2008.180-
dc.citation.titleGene therapy-
dc.citation.volume16-
dc.citation.number3-
dc.citation.date2009-
dc.citation.startPage415-
dc.citation.endPage425-
dc.identifier.bibliographicCitationGene therapy, 16(3). : 415-425, 2009-
dc.identifier.eissn1476-5462-
dc.relation.journalidJ009697128-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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