25 268

Cited 0 times in

Mouse monoclonal autoantibodies penetrate mouse macrophage cells and stimulate NF-kappaB activation and TNF-alpha release.

Authors
Jang, EJ; Nahm, DH; Jang, YJ
Citation
Immunology letters, 124(2):70-76, 2009
Journal Title
Immunology letters
ISSN
0165-24781879-0542
Abstract
Autoantibodies against double-stranded DNA (dsDNA) are found in the serum of systemic lupus erythematosus (SLE) patients. However, the mechanism by which anti-dsDNA antibodies (Abs) contribute to the pathogenesis of SLE is not yet fully understood. In this study, we investigated four anti-dsDNA mouse monoclonal autoantibodies that share positively charged amino acids (including arginines) in their complementarity determining regions for their ability to penetrate RAW264.7 macrophage cells, activate NF-kappaB and stimulate TNF-alpha production. All four antibodies penetrated into macrophage cells and increased the level of extracellular TNF-alpha; two also activated NF-kappaB. The fact that two of four cell-penetrating anti-dsDNA mAbs induced both NF-kappaB activation and TNF-alpha production in macrophages suggests that at least some autoantibodies against dsDNA may play a role in the pathogenesis of SLE by penetrating into macrophage cells and nuclei, and subsequently inducing the pro-inflammatory cytokine, TNF-alpha, by binding to the NF-kappaB gene and stimulating its transcriptional activity.
MeSH terms
Amino Acid SequenceAnimalsAntibodies, Antinuclear/immunology*Antibodies, Antinuclear/metabolismAntibodies, Monoclonal/immunologyAntibodies, Monoclonal/metabolismCell LineDNA/immunologyDNA/metabolismHumansImmunologic Factors/pharmacologyLupus Erythematosus, Systemic/geneticsLupus Erythematosus, Systemic/immunology*Macrophages/immunology*Macrophages/metabolismMiceMolecular Sequence DataNF-kappa B/biosynthesis*NF-kappa B/geneticsTranscription, GeneticTumor Necrosis Factor-alpha/biosynthesis*Tumor Necrosis Factor-alpha/genetics
DOI
10.1016/j.imlet.2009.04.005
PMID
19394364
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
남, 동호장, 영주
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse