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Inactivation patterns of p16/INK4A in oral squamous cell carcinomas.

Authors
Lee, JK; Kim, MJ; Hong, SP; Hong, SD
Citation
Experimental & molecular medicine, 36(2):165-171, 2004
Journal Title
Experimental & molecular medicine
ISSN
1226-36132092-6413
Abstract
The p16/INK4A is one of the major target genes in carcinogenesis and its inactivation has frequently been reported in other types of tumors. The purpose of this study is to evaluate inactivation patterns of p16/INK4A in oral squamous cell carcinoma. Six different oral cancer cell lines, SCC-4, SCC-9, SCC-15, SCC-25, KB, and SNUDH- 379 were examined for inactivation of p16/INK4A genes. In the analysis of p16/INK4A gene inactivation, PCR amplification, direct sequencing, and methylation-specific PCR methods were adopted for evaluation of homozygous deletion, point mutation, and promoter hypermethylation, respectively. Homozygous deletion was detected in SCC-25 and SCC-9. SCC-15 showed hypermethylated promoter region within p16/INK4A gene. It is suggestive in the present study that inactivation patterns of p16/INK4A were mainly homozygous deletion, promoter methylation rather than point mutation in oral squamous cancer cell lines, so treatment modalities of oral squamous cell carcinoma should be focused on these types of inactivation.
MeSH terms
Carcinoma, Squamous Cell/*geneticsCell Line, TumorCyclin-Dependent Kinase Inhibitor p16/*geneticsDNA Methylation*Gene SilencingHomozygoteHumansMouth Neoplasms/*geneticsPoint MutationPromoter Regions, Genetic/genetics
DOI
10.1038/emm.2004.23
PMID
15150445
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Journal Papers > School of Medicine / Graduate School of Medicine > Dentistry
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