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Study on identification of RIPK1 inhibitor for therapeutic potential : Identification of novel inhibitor of receptor-interacting protein kinase 1 (RIPK1)
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김, 유선 | - |
| dc.contributor.author | 김, 병주 | - |
| dc.date.accessioned | 2025-01-17T06:43:35Z | - |
| dc.date.available | 2025-01-17T06:43:35Z | - |
| dc.date.issued | 2024 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/33740 | - |
| dc.language.iso | ko | - |
| dc.title | Study on identification of RIPK1 inhibitor for therapeutic potential : Identification of novel inhibitor of receptor-interacting protein kinase 1 (RIPK1) | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000033970 | - |
| dc.subject.keyword | RIPK1 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | 김, 병주 | - |
| dc.contributor.affiliatedAuthor | 김, 유선 | - |
| dc.date.awarded | 2024 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2024 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
| dc.description.tableOfContents | I. INTRODUCTION 1
II. MATERIALS AND METHODS 4 A. Antibodies and chemical reagents 4 B. Cell lines and culture conditions 5 C. Immunoblot analysis and immunoprecipitation 5 D. Plasmid construction and transfection 6 E. Cytotoxicity assays 6 F. Immunofluorescence staining 6 G. Primary culture and activation of BMDMs 6 H. Flow cytometry 7 I. Quantitative RT-PCR 7 J. Histology analysis 7 K. Serum biochemistry 8 L. LPS-induced SIRS model 8 M. Structural similarity calculation 8 N. Molecular docking and dynamics simulation study 8 O. Statistical analysis 9 III. RESULTS 10 A. Identification of phensuximide as a potential RIPK 1 inhibitor. 10 B. Phensuximide shows potent inhibitory effect on TNF-induced necroptotic cell death. 15 C. Inhibition of RIPK1 kinase activity by phensuximide treatment. 23 D. Phensuximide does not involved in receptor-mediated complex I signal. 33 E. Inhibition of RIPK1 kinase activity-dependent cell death and inflammatory response by phensuximide in macrophages. 38 F. Phensuximide is a potential therapeutic candidate for necroptosis related diseases. 46 IV. DISCUSSION 52 V. REFERENCES 54 국문요약 59 | - |
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