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Metalloproteinase-9 is increased after toluene diisocyanate exposure in the induced sputum from patients with toluene diisocyanate-induced asthma.

Authors
Park, HS; Kim, HA; Jung, JW; Kim, YK; Lee, SK; Kim, SS; Nahm, DH
Citation
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 33(1):113-118, 2003
Journal Title
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
ISSN
0954-78941365-2222
Abstract
BACKGROUND AND OBJECTIVE: Persistent asthma symptoms are associated with airway inflammation and remodeling, which may be mediated through metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP). The aim of this study was to evaluate MMPs and TIMP involvement in toluene diisocyanate (TDI)-induced asthma.



MATERIALS AND METHOD: Induced sputum was collected in eight newly diagnosed TDI-induced asthma subjects (group I) before and 7 h after the TDI and placebo challenges and in 12 subjects with TDI-induced occupational asthma diagnosed 5 years previously with persistent asthma symptoms (group II). Sera was collected in group I at diagnosis, and in group II, they were collected at the time of the study. 12 nonasthmatic healthy subjects were enrolled as controls. MMP-9, MMP-2 and TIMP-1 levels in both sputum and serum were measured by enzyme-linked immunosorbent assay (ELISA). Gelatinase activity in the sputum was confirmed by zymographic analysis.



RESULTS: The serum TIMP-1 level was significantly higher in asthma patients than in healthy controls (P = 0.01), while MMP-9 level was significantly lower in asthmatic patients (P = 0.03). There was no significant difference in MMP-2 level (P = 0.27). MMP-9 level in the sputum was significantly increased after the TDI challenges (P = 0.01). TIMP-1 level in sputum tended to increase after TDI challenges, but no statistical significance was noted (P = 0.09). MMP-9 and MMP-9/TIMP-1 levels in the sputum were significantly higher in group II than in group I (P = 0.04, P = 0.02) with no significant difference in TIMP-1 level. Minimal amount of MMP-2 was found in sputum. Zymography demonstrated that MMP-9 level increased and active form of MMP-9 was generated after the TDI bronchoprovocation test.



CONCLUSION: TDI exposure leads to overproduction of MMP-9, which may induce airway inflammation and remodeling, and then contribute to persistent asthmatic symptoms in TDI-induced asthma.
MeSH terms
AdultAsthma/chemically induced*Asthma/enzymologyBronchial Provocation TestsBronchoconstrictor Agents/diagnostic useCase-Control StudiesFemaleGelatinases/analysisHumansIrritants/adverse effects*Irritants/diagnostic useLung/physiopathologyMaleMatrix Metalloproteinase 2/analysisMatrix Metalloproteinase 9/analysis*Matrix Metalloproteinase 9/bloodMethacholine Chloride/diagnostic useMiddle AgedOccupational Diseases/chemically induced*Occupational Diseases/enzymologySputum/chemistry*Tissue Inhibitor of Metalloproteinase-1/analysisTissue Inhibitor of Metalloproteinase-1/bloodToluene 2,4-Diisocyanate/adverse effects*Toluene 2,4-Diisocyanate/diagnostic use
PMID
12534559
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
AJOU Authors
박, 해심남, 동호
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