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Expression of hepatitis B virus X (HBx) gene is up-regulated by adriamycin at the post-transcriptional level.

Authors
Yun, C; Lee, JH; Wang, JH; Seong, JK; Oh, SH; Yu, DY; Cho, H
Citation
Biochemical and biophysical research communications, 296(5):1157-1163, 2002
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
Hepatitis B virus (HBV) X protein (HBx) is thought to be involved in the development of liver cancer and alteration of cellular HBx level may influence the pathological progression of HBV-induced liver diseases. We found that the cellular levels of HBx mRNA transcript and protein in cells were greatly enhanced by adriamycin, a topoisomerase II inhibitor. Up-regulation of HBx mRNA by adriamycin was also observed in HBx transgenic mice, which was accompanied with a significant increase of VEGF mRNA, the downstream target of HBx. When we investigated the underlying mechanism, we found that half-life of HBx mRNA in HBx-expressing Chang cells was about 3h, but was prolonged to >6h in the presence of adriamycin. Moreover, half-life of rapidly degrading HBx protein was determined as about 15min however, it remained almost constant until 60min in the presence of adriamycin. These results provide the first evidence that the cellular level of HBx gene can be increased at the post-transcriptional level.
MeSH terms
AnimalsCell LineDoxorubicin/*pharmacologyEnzyme Inhibitors/*pharmacologyGene Expression Regulation, Viral/*drug effectsKineticsMiceMice, Inbred C57BLMice, Transgenic*RNA StabilityRNA, Messenger/metabolismRNA, Viral/metabolism*Topoisomerase II InhibitorsTrans-Activators/*biosynthesis/geneticsTranscription, GeneticUp-Regulation
PMID
12207895
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
윤, 차원이, 재호조, 혜성
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