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Hepatitis B virus X protein induced expression of interleukin 18 (IL-18): a potential mechanism for liver injury caused by hepatitis B virus (HBV) infection.

DC Field Value Language
dc.contributor.authorLee, MO-
dc.contributor.authorChoi, YH-
dc.contributor.authorShin, EC-
dc.contributor.authorKang, HJ-
dc.contributor.authorKim, YM-
dc.contributor.authorJeong, SY-
dc.contributor.authorSeong, JK-
dc.contributor.authorYu, DY-
dc.contributor.authorCho, H-
dc.contributor.authorPark, JH-
dc.contributor.authorKim, SJ-
dc.date.accessioned2011-07-18T07:08:38Z-
dc.date.available2011-07-18T07:08:38Z-
dc.date.issued2002-
dc.identifier.issn0168-8278-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3420-
dc.description.abstractBACKGROUND/AIMS: The hepatitis B virus X protein (HBx), a major viral transactivator, is implicated in hepatic inflammation, since it induces many pro-inflammatory cytokines at transcriptional level. The aim of this study was to investigate role of HBx in expression of interleukin 18 (IL-18), a newly identified cytokine that up-regulates Fas ligand (FasL) expression.



METHODS: Chang X-34 that expressing HBx under the control of a doxycycline-inducible promoter, and hepatitis B virus (HBV)-integrated hepatoma cell lines were examined for IL-18 expression by Northern and Western blotting analysis. To test the role of IL-18 produced by hepatoma cells, FasL expression was examined by flow cytometry after treatment with neutralizing anti-IL-18 antibodies. Further, IL-18 expression was examined in the liver tissues of HBx-transgenic mice.



RESULTS: Induction of IL-18 following HBx expression in Chang X-34 and the pattern of IL-18 expression in HBV-integrated cell lines, implicated that HBx transcriptionally induces IL-18 expression. Neutralizing anti-IL-18 antibodies blocked the expression of FasL, suggesting that IL-18 plays a critical role in FasL expression. Further, IL-18 expression in the HBx-transgenic liver, was correlated with the degree of hepatitis.



CONCLUSIONS: Our results demonstrated that HBx induces IL-18 expression in liver, which may be associated with hepatic injury by amplifying FasL expression during HBV infection.
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dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHCarcinoma, Hepatocellular-
dc.subject.MESHFas Ligand Protein-
dc.subject.MESHGene Expression Regulation, Viral-
dc.subject.MESHHepatitis B virus/*physiology-
dc.subject.MESHHepatitis B, Chronic/*immunology/pathology/*virology-
dc.subject.MESHHepatocytes/immunology/pathology/virology-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-18/*genetics/secretion-
dc.subject.MESHLiver/immunology/pathology/virology-
dc.subject.MESHLiver Neoplasms-
dc.subject.MESHMembrane Glycoproteins/genetics-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHTrans-Activators/*genetics-
dc.subject.MESHTranscription, Genetic/physiology-
dc.subject.MESHTumor Cells, Cultured-
dc.titleHepatitis B virus X protein induced expression of interleukin 18 (IL-18): a potential mechanism for liver injury caused by hepatitis B virus (HBV) infection.-
dc.typeArticle-
dc.identifier.pmid12175634-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0168827802001812-
dc.contributor.affiliatedAuthor조, 혜성-
dc.type.localJournal Papers-
dc.citation.titleJournal of hepatology-
dc.citation.volume37-
dc.citation.number3-
dc.citation.date2002-
dc.citation.startPage380-
dc.citation.endPage386-
dc.identifier.bibliographicCitationJournal of hepatology, 37(3):380-386, 2002-
dc.identifier.eissn1600-0641-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology

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