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Interleukin-13 and -4 induce death of activated microglia.

Yang, MS; Park, EJ; Sohn, S; Kwon, HJ; Shin, WH; Pyo, HK; Jin, B; Choi, KS; Jou, I; Joe, EH
Glia, 38(4):273-280, 2002
Journal Title
When the brain suffers injury, microglia migrate to the damaged sites and become activated. These activated microglia are not detected several days later and the mechanisms underlying their disappearance are not well characterized. In this study, we demonstrate that interleukin (IL)-13, an anti-inflammatory cytokine, selectively induces cell death of activated microglia in vitro. Cell death was detected 4 days after the coaddition of IL-13 with any one of the microglial activators, lipopolysaccharide (LPS), ganglioside, or thrombin. This cell death occurred in a time-dependent manner. LPS, ganglioside, thrombin, or IL-13 alone did not induce cell death. Among anti-inflammatory cytokines, IL-4 mimicked the effect of IL-13, while TGF-beta did not. Cells treated with IL-13 plus LPS, or IL-13 plus ganglioside, showed the characteristics of apoptosis when analyzed by electron microscopy and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining. Electron micrographs also showed microglia engulfing neighboring dead cells. We propose that IL-13 and IL-4 induce death of activated microglia, and that this process is important for prevention of chronic inflammation that can cause tissue damage.
MeSH terms
AnimalsAnimals, NewbornBrain Injuries/immunology/*metabolism/physiopathologyCell Death/drug effects/*immunologyCell Size/drug effects/physiologyCells, CulturedEncephalitis/immunology/*metabolism/physiopathologyEthidium/*analogs & derivatives/diagnostic useFluoresceins/diagnostic useFluorescent Dyes/diagnostic useGangliosides/pharmacologyGliosis/immunology/*metabolism/physiopathologyIn Situ Nick-End LabelingIntercalating Agents/diagnostic useInterleukin-13/*metabolism/pharmacologyInterleukin-4/*metabolism/pharmacologyLipopolysaccharides/pharmacologyMicroglia/drug effects/*metabolism/ultrastructureMicroscopy, ElectronRatsRats, Sprague-DawleyTransforming Growth Factor beta/metabolism/pharmacology
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Journal Papers > Research Organization > Chronic Inflammatory Disease Research Center
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Journal Papers > Research Organization > Institute for Medical Sciences
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
박, 은정손, 성향진, 병관최, 경숙주, 일로조, 은혜
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