78 220

Cited 0 times in

Analysis of mitochondrial free radical generation in animal models of neuronal disease.

Authors
Kim, DY; Won, SJ; Gwag, BJ
Citation
Free radical biology & medicine, 33(5):715-723, 2002
Journal Title
Free radical biology & medicine
ISSN
0891-58491873-4596
Abstract
Mitochondria, the power plant of all eukaryotic cells, produce cellular energy in the form of ATP via electron transport and oxidative phosphorylation. However, the mitochondria leak electrons that can act as major sources of oxidative stress, and their dysfunction, have been proposed as causative events underlying neurodegeneration in stroke and neurodegenerative diseases. We examined whether MitoTracker Red CM-H(2)XRos, a rosamine derivative used to detect mitochondrial free radicals in vitro, would be applied to analyze the mitochondrial free radicals in various models of neurological diseases in vivo. The injections of MitoTracker Red CM-H(2)XRos revealed generation of mitochondrial free radicals primarily in vulnerable neurons following focal cerebral ischemia as well as administration of Fe(2+) or 3-nitropropionic acid. MitoTracker Red CM-H(2)XRos was retained after fixation, compatible with immunocytochemistry or nuclear staining, and can be applied to study roles of mitochondrial free radicals in the process of neurodegeneration in vivo.
MeSH terms
Adenosine Triphosphate/metabolismAnimalsBrain Ischemia/*metabolismDisease Models, AnimalFluorescent Dyes/pharmacology*Free RadicalsHumansHuntington Disease/metabolismImmunohistochemistryIron/metabolismMaleMitochondria/*metabolismNeurodegenerative Diseases/*metabolismNeurons/metabolismNitro CompoundsOrganic ChemicalsPropionic Acids/metabolismRatsRats, Sprague-Dawley
PMID
12208358
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Science
AJOU Authors
원, 석준곽, 병주
Full Text Link
Files in This Item:
Full-Text Not Available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse