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JAK-STAT signaling mediates gangliosides-induced inflammatory responses in brain microglial cells.

Authors
Kim, OS; Park, EJ; Joe, EH; Jou, I
Citation
The Journal of biological chemistry, 277(43):40594-40601, 2002
Journal Title
The Journal of biological chemistry
ISSN
0021-92581083-351X
Abstract
Neuronal cell membranes are particularly rich in gangliosides, which play important roles in brain physiology and pathology. Previously, we reported that gangliosides could act as microglial activators and are thus likely to participate in many neuronal diseases. In the present study we provide evidence that JAK-STAT inflammatory signaling mediates gangliosides-stimulated microglial activation. Both in rat primary microglia and murine BV2 microglial cells, gangliosides stimulated nuclear factor binding to GAS/ISRE elements, which are known to be STAT-binding sites. Consistent with this, gangliosides rapidly activated JAK1 and JAK2 and induced phosphorylation of STAT1 and STAT3. In addition, gangliosides increased transcription of the inflammation-associated genes inducible nitric-oxide synthase, ICAM-1, and MCP-1, which are reported to contain STAT-binding elements in their promoter regions. AG490, a JAK inhibitor, reduced induction of these genes, nuclear factor binding activity, and activation of STAT1 and -3 in gangliosides-treated microglia. AG490 also inhibited gangliosides-induced release of nitric oxide, an inflammation hallmark. Furthermore, AG490 markedly reduced activation of ERK1/2 MAPK, indicating that ERKs act downstream of JAK-STAT signaling during microglial activation. However, AG490 did not affect activation of p38 MAPK. We also report that the sialic acid residues present on gangliosides may be one of the essential components in activation of JAK-STAT signaling. The present study indicates that JAK-STAT signaling is an early event in gangliosides-induced brain inflammatory responses.
MeSH terms
AnimalsBase SequenceBrain/*metabolism/pathologyCells, CulturedDNA PrimersDNA-Binding Proteins/*metabolismElectrophoretic Mobility Shift AssayEnzyme ActivationGangliosides/*metabolismInflammation Mediators/*metabolismMicroglia/*metabolism/pathologyN-Acetylneuraminic Acid/metabolismNuclear Proteins/metabolismProtein BindingProtein-Tyrosine Kinases/*metabolismRatsRats, Sprague-DawleySTAT1 Transcription FactorSTAT3 Transcription Factor*Signal TransductionTrans-Activators/*metabolismTyrphostins/pharmacology
DOI
10.1074/jbc.M203885200
PMID
12191995
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Journal Papers > Research Organization > Chronic Inflammatory Disease Research Center
AJOU Authors
박, 은정조, 은혜주, 일로
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