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Brain-derived neurotrophic factor can act as a pronecrotic factor through transcriptional and translational activation of NADPH oxidase.

Authors
Kim, SH; Won, SJ; Sohn, S; Kwon, HJ; Lee, JY; Park, JH; Gwag, BJ
Citation
The Journal of cell biology, 159(5):821-831, 2002
Journal Title
The Journal of cell biology
ISSN
0021-95251540-8140
Abstract
Several lines of evidence suggest that neurotrophins (NTs) potentiate or cause neuronal injury under various pathological conditions. Since NTs enhance survival and differentiation of cultured neurons in serum or defined media containing antioxidants, we set out experiments to delineate the patterns and underlying mechanisms of brain-derived neurotrophic factor (BDNF)-induced neuronal injury in mixed cortical cell cultures containing glia and neurons in serum-free media without antioxidants, where the three major routes of neuronal cell death, oxidative stress, excitotoxicity, and apoptosis, have been extensively studied. Rat cortical cell cultures, after prolonged exposure to NTs, underwent widespread neuronal necrosis. BDNF-induced neuronal necrosis was accompanied by reactive oxygen species (ROS) production and was dependent on the macromolecular synthesis. cDNA microarray analysis revealed that BDNF increased the expression of cytochrome b558, the plasma membrane-spanning subunit of NADPH oxidase. The expression and activation of NADPH oxidase were increased after exposure to BDNF. The selective inhibitors of NADPH oxidase prevented BDNF-induced ROS production and neuronal death without blocking antiapoptosis action of BDNF. The present study suggests that BDNF-induced expression and activation of NADPH oxidase cause oxidative neuronal necrosis and that the neurotrophic effects of NTs can be maximized under blockade of the pronecrotic action.
MeSH terms
AnimalsBrain-Derived Neurotrophic Factor/*pharmacology/physiologyCell Death/drug effectsCells, CulturedCerebral Cortex/cytology/drug effects/*enzymologyCoculture TechniquesCorpus Striatum/cytologyCytochrome b Group/metabolismEnzyme ActivationEnzyme Inhibitors/pharmacology*Gene Expression Regulation, EnzymologicMaleNADPH Oxidase/chemistry/*genetics/metabolism*NecrosisNeuroglia/cytologyNeurons/*enzymology/metabolism/ultrastructureOxidative Stress/drug effectsProtein BiosynthesisRNA, Messenger/metabolismRatsRats, Sprague-DawleyReactive Oxygen Species/metabolismTranscription, Genetic
DOI
10.1083/jcb.200112131
PMID
12460985
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Science
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
김, 선희원, 석준손, 성향곽, 병주
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