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Exposure to toluene diisocyanate (TDI) induces IL-8 production from bronchial epithelial cells: effect of pro-inflammatory cytokines.

Lee, YM; Kim, HA; Park, HS; Lee, SK; Nahm, DH
Journal of Korean medical science, 18(6):809-812, 2003
Journal Title
Journal of Korean medical science
This investigation was designed to confirm IL-8 production from human bronchial epithelial cells with toluene diisocyanate (TDI) exposure and to examine the effects of pro-inflammatory cytokine and dexamethasone. We cultured Beas-2B, a bronchial epithelial cell line with TDI-HSA conjugate and compared with those without conjugate. IL-8 in the supernatant was measured by ELISA. To evaluate the effect of proinflammatory cytokines, peripheral blood mononuclear cells (PBMC) were collected from TDI- and non-TDI asthma patients, and were added to the epithelial cell culture. Dexamethasone or antibodies to TNF-alpha and IL-1beta were pre-incubated with PBMC supernatant. There was a significant production of IL-8 from bronchial epithelial cells with addition of TDI-HSA conjugate in a dose-dependent manner, which was significantly augmented with addition of PBMC supernatant. Higher production of IL-8 was noted with addition of PBMC supernatant from TDI-asthma patients than in those from non-TDI asthma patients. IL-1beta and IL-1beta/TNF alpha antibodies were able to suppress the IL-8 productions. Pre-treatment of dexamethasone induced dose-dependent inhibition of the IL-8 production. These results suggest that the IL-8 production from bronchial epithelial cells contribute to neutrophil recruitment occurring in TDI-induced airway inflammation. IL-1beta released from PBMC of TDI-induced asthma patients may be one of the pro-inflammatory cytokines to enhance IL-8 production.
MeSH terms
Asthma/immunologyBronchi/cytology*Bronchi/metabolismCell LineDexamethasone/pharmacologyEpithelial Cells/cytologyEpithelial Cells/drug effects*Epithelial Cells/metabolism*Glucocorticoids/pharmacologyHumansInterleukin-8/metabolism*Leukocytes, Mononuclear/cytologyLeukocytes, Mononuclear/metabolismToluene 2,4-Diisocyanate/toxicity*
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Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
AJOU Authors
박, 해심남, 동호
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