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RNAi methodologies for the functional study of signaling molecules.

Authors
Lee, G; Santat, LA; Chang, MS; Choi, S
Citation
PloS one, 4(2):e4559-e4559, 2009
Journal Title
PloS one
ISSN
1932-6203
Abstract
RNA interference (RNAi) was investigated with the aim of achieving gene silencing with diverse RNAi platforms that include small interfering RNA (siRNA), short hairpin RNA (shRNA) and antisense oligonucleotides (ASO). Different versions of each system were used to silence the expression of specific subunits of the heterotrimeric signal transducing G-proteins, G alpha i2 and G beta 2, in the RAW 264.7 murine macrophage cell line. The specificity of the different RNA interference (RNAi) platforms was assessed by DNA microarray analysis. Reliable RNAi methodologies against the genes of interest were then developed and applied to functional studies of signaling networks. This study demonstrates a successful knockdown of target genes and shows the potential of RNAi for use in functional studies of signaling molecules.
MeSH terms
AnimalsBiomedical Research/methodsCell LineGene Silencing*MacrophagesMethodsMiceOligonucleotide Array Sequence Analysis*RNA InterferenceRNA, Small InterferingSignal Transduction/genetics*
DOI
10.1371/journal.pone.0004559
PMID
19238203
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Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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