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Bone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells.

DC Field Value Language
dc.contributor.authorKim, DJ-
dc.contributor.authorMoon, SH-
dc.contributor.authorKim, H-
dc.contributor.authorKwon, UH-
dc.contributor.authorPark, MS-
dc.contributor.authorHan, KJ-
dc.contributor.authorHahn, SB-
dc.contributor.authorLee, HM-
dc.date.accessioned2011-07-22T02:07:03Z-
dc.date.available2011-07-22T02:07:03Z-
dc.date.issued2003-
dc.identifier.issn0362-2436-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3525-
dc.description.abstractSTUDY DESIGN: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and human intervertebral disc (IVD) cells.



OBJECTIVES: To demonstrate the effect of BMP-2 on mRNAs expression (collagen type I, collagen type II, aggrecan, and osteocalcin), proteoglycan synthesis, expression of alkaline phosphatase, bone nodule formation in human IVD cells.



SUMMARY OF BACKGROUND DATA: BMP-2 was widely known as a powerful agent for osteoinduction and a crucial growth factor for early chondrogenesis and maintenance of cartilaginous phenotype. BMP-2 proved to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells. Nevertheless, the effect of BMP-2 on IVD cells, whether chondrogenic or osteogenic, was not thoroughly elucidated in transcriptional level and histochemical stains.



MATERIALS AND METHODS: Human IVDs were harvested and enzymatically digested. Then IVD cells were cultured three-dimensionally in alginate beads. Osteoblasts were cultured from cancellous bone of ilium for histochemical stains. Recombinant human BMP-2 (rhBMP-2) was produced by Chinese hamster ovary cells after transduction of BMP-2 cDNA, then concentrated and purified. Then IVD cell cultures were exposed to various concentrations of rhBMP-2. Reverse transcription-polymerase chain reaction for mRNA expression of aggrecan, collagen type I, collagen type II, and osteocalcin was performed. Newly synthesized proteoglycan was measured by 35S-sulfate incorporation on Sephadex G-25 M in PD 10 columns. As a histochemical examination, alkaline phosphatase and Alizarin red-S stains were used to detect osteogenic marker and bone nodule formation, respectively.



RESULTS: In the rhBMP-2 treated cultures, there was increased newly synthesized proteoglycan (67% in 300 ng/mL and 200% in 1,500 ng/mL of rhBMP-2) and up-regulated expression of aggrecan, collagen type I, and collagen type II mRNA over untreated control. However, rhBMP-2 did not up-regulate expression of osteocalcin mRNA in the given dose and culture period. IVD cell cultures with rhBMP-2 showed no evidence of bone formation in histochemical stains, i.e., alkaline phosphatase and Alizarin red-S, while osteoblast culture exhibited strong positive stains.



CONCLUSIONS: The rhBMP-2 clearly up-regulated mRNA expression of chondrogenic components and also stimulated proteoglycan synthesis without expression of osteogenic phenotype. Taken together, this study raise the possibility of rhBMP-2 can be anabolic agent for regenerating matrix of intervertebral disc.
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dc.language.isoen-
dc.subject.MESHAdult-
dc.subject.MESHAggrecans-
dc.subject.MESHAlkaline Phosphatase-
dc.subject.MESHAnthraquinones-
dc.subject.MESHBone Morphogenetic Protein 2-
dc.subject.MESHBone Morphogenetic Proteins-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChondrocytes-
dc.subject.MESHCollagen Type I-
dc.subject.MESHCollagen Type II-
dc.subject.MESHColoring Agents-
dc.subject.MESHExtracellular Matrix Proteins-
dc.subject.MESHGene Expression-
dc.subject.MESHHumans-
dc.subject.MESHIntervertebral Disk-
dc.subject.MESHLectins, C-Type-
dc.subject.MESHMiddle Aged-
dc.subject.MESHOsteoblasts-
dc.subject.MESHOsteocalcin-
dc.subject.MESHPhenotype-
dc.subject.MESHProteoglycans-
dc.subject.MESHRNA, Messenger-
dc.subject.MESHRecombinant Proteins-
dc.subject.MESHTransforming Growth Factor beta-
dc.titleBone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells.-
dc.typeArticle-
dc.identifier.pmid14673369-
dc.identifier.urlhttp://meta.wkhealth.com/pt/pt-core/template-journal/lwwgateway/media/landingpage.htm?issn=0362-2436&volume=28&issue=24&spage=2679-
dc.contributor.affiliatedAuthor한, 경진-
dc.type.localJournal Papers-
dc.identifier.doi10.1097/01.BRS.0000101445.46487.16-
dc.citation.titleSpine-
dc.citation.volume28-
dc.citation.number24-
dc.citation.date2003-
dc.citation.startPage2679-
dc.citation.endPage2684-
dc.identifier.bibliographicCitationSpine, 28(24). : 2679-2684, 2003-
dc.identifier.eissn1528-1159-
dc.relation.journalidJ003622436-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
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