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Induction of radioprotective peroxiredoxin-I by ionizing irradiation.

Authors
Chen, WC; McBride, WH; Iwamoto, KS; Barber, CL; Wang, CC; Oh, YT; Liao, YP; Hong, JH; de Vellis, J; Shau, H
Citation
Journal of neuroscience research, 70(6):794-798, 2002
Journal Title
Journal of neuroscience research
ISSN
0360-40121097-4547
Abstract
Results of this study indicate a radioprotective effect of peroxiredoxin-I. Peroxiredoxin-I is an antioxidant that scavenges hydroperoxides, whereas reactive oxygen species are the main mediators of ionizing radiation toxicity. We hypothesized that peroxiredoxin-I might be induced by cellular exposure to radiation and act to protect them against its cytotoxic effects. Western blot and Northern blot analyses were used to assess peroxiredoxin-I protein and mRNA expression. Rat C6 glioma cells were engineered to overexpress sense or antisense human peroxiredoxin-I using retroviral vectors. Clonogenic cell survival was used to assess radiosensitivities of the engineered cells. Ionizing radiation induced peroxiredoxin-I protein and mRNA expression in human HT29 colon cancer and rat C6 glioma cells in a dose- and time-dependent manner over a 24 hr period. To determine the effect of peroxiredoxin-I on radiation responses, C6 glioma cells were engineered to overexpress sense or antisense human peroxiredoxin-I. In clonogenic assays, cells overexpressing peroxiredoxin-I were more radioresistant. Cells transduced with antisense peroxiredoxin-I were marginally more sensitive to radiation toxicity. Irradiation can induce peroxiredoxin-I expression, and the increased peroxiredoxin-I may protect cells from further radiation damage. These results suggest that protection by peroxiredoxin-I may play an important role in the survival of glioma and colon cancer cells in patients undergoing radiation therapy.
MeSH terms
AnimalsBlotting, NorthernBlotting, WesternCell Survival/radiation effectsDose-Response Relationship, RadiationGenetic EngineeringGlioma/geneticsHT29 CellsHumansPeroxidases/genetics/*metabolism/*radiation effectsPeroxiredoxinsRNA, Messenger/*radiation effects*Radiation ToleranceRadiation, IonizingRatsTumor Cells, Cultured
DOI
10.1002/jnr.10435
PMID
12444601
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Radiation Oncology
AJOU Authors
오, 영택
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