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Analysis of changes in the viability and gene expression profiles of human mesenchymal stromal cells over time.

Authors
Lee, KA; Shim, W; Paik, MJ; Lee, SC; Shin, JY; Ahn, YH; Park, K; Kim, JH; Choi, S; Lee, G
Citation
Cytotherapy, 11(6):688-697, 2009
Journal Title
Cytotherapy
ISSN
1465-32491477-2566
Abstract
BACKGROUND AIMS: Because of their ability to differentiate and widespread availability, human mesenchymal stromal cells (hMSC) are often used as a clinical therapeutic tool. However, the factors that determine the quality and viability of hMSC are not well understood. METHODS: We evaluated the viability of hMSC over time using flow cytometry analysis (FACS) and transmission electron microscopy (TEM) to determine if morphologic changes occurred in hMSC. In addition, we conducted gene expression prof ling using an Affymetrix Human Genome U133 Plus 2.0 Array. RESULTS: FACS analysis revealed that 83% and 76% of the cells were viable in sterilized phosphate-buffered saline (PBS) after 6 h and 12 h, respectively. TEM data revealed that the total number of cells with healthy chromatins or a few cytosolic vacuoles was significantly reduced over time. We then conducted gene expression profiling using a microarray, which revealed changes in the expression of 2949 functional genes. Specifically, among the total of 50,000 gene probes evaluated, the expression levels of apoptosis and stress-related genes were significantly increased over time. CONCLUSIONS: The results of this study suggest that the viability of hMSC decreases after disassociation from the culture dish and time is an essential factor when considering hMSC as a potential source for stem cell-based direct transplantation.
MeSH terms
Cell Differentiation/geneticsCell Differentiation/physiologyCell SeparationCell Survival/physiology*Down-Regulation/geneticsDown-Regulation/physiologyFlow CytometryGene Expression ProfilingHumansMesenchymal Stem Cells/physiology*Mesenchymal Stem Cells/ultrastructureMicroscopy, Electron, TransmissionUp-Regulation/geneticsUp-Regulation/physiology
DOI
10.3109/14653240902974032
PMID
19878055
Appears in Collections:
Journal Papers > Research Organization > Institute for Neuroregeneration & Stem Cell Research
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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