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A well-defined in vitro three-dimensional culture of human endometrium and its applicability to endometrial cancer invasion.

Authors
Park, DW; Choi, DS; Ryu, HS; Kwon, HC; Joo, H; Min, CK
Citation
Cancer letters, 195(2):185-192, 2003
Journal Title
Cancer letters
ISSN
0304-38351872-7980
Abstract
A three-dimensional (3-D) endometrium culture was established, in which human endometrial stromal cells embedded in a mixture of collagen I, a major component of extracellular matrix, and matrigel, a basement membrane material, supports the epithelial cells seeded on top of the collagen/matrigel matrix. The biological growth and differentiation of the epithelial cells were studied microscopically and immunohistochemically. Transmission electron microscopy showed a polarized columnar epithelium in monolayer with basally positioned nuclei. Scanning electron microscopy revealed a confluent epithelium with an abundance of microvilli and cilia as well as pinopodes on the apical surface. An immunohistochemical staining showed that integrin alpha1, alpha4, and beta3 were co-localized with cytokeratin, confirming the epithelial origin of the cells. In contrast, immunoreactivity against cyclooxygenase-1 or -2 was positive in both epithelial and stromal cells. When epithelial cells were replaced by KLE cells, an endometrial cancer cell of epithelial origin, invasion of KLE cells into the stromal fraction was observed. The invasion was closely correlated to expression of matrix metalloproteinases and their tissue inhibitors of metalloproteinases in a manner consistent with paracrine fashion. The present 3-D culture imitates the normal endometrium physiologically as well as morphologically, thus provides an excellent in vitro tissue suitable for reproducing in vivo physiological processes, including endometrial cancer invasion.
MeSH terms
Cell CommunicationCell DifferentiationCells, Cultured/chemistryCells, Cultured/cytologyCollagenCollagen Type ICulture MediaCulture Techniques/methods*Cyclooxygenase 1Cyclooxygenase 2Drug CombinationsEndometrial Neoplasms/pathology*Endometrium/chemistryEndometrium/cytology*Epithelial Cells/chemistryEpithelial Cells/cytologyFemaleHumansIntegrin alpha1/analysisIntegrin alpha4/analysisIntegrin beta3/analysisIsoenzymes/analysisKeratins/analysisLamininMatrix Metalloproteinases/analysisMembrane ProteinsMicroscopy, ElectronMicroscopy, Electron, ScanningNeoplasm InvasivenessNeoplasm Proteins/analysisProstaglandin-Endoperoxide Synthases/analysisProteoglycansStromal Cells/chemistryStromal Cells/cytologyTissue Inhibitor of Metalloproteinase-1/analysisTissue Inhibitor of Metalloproteinase-2/analysis
PMID
12767527
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Obstetrics & Gynecology
AJOU Authors
유, 희석
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