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Simultaneous expression of allogenic class II MHC and B7.1 (CD80) molecules in A20 B-lymphoma cell line enhances tumor immunogenicity.

Authors
Jang, YJ; Nam, SY; Kim, MS; Seong, RH; Park, YS; Chung, YH; Chung, HY
Citation
Molecules and cells, 13(1):130-136, 2002
Journal Title
Molecules and cells
ISSN
1016-84780219-1032
Abstract
We expressed the allogenic class II MHC antigen and B7.1 (CD80) co-stimulatory molecule in A20 beta-lymphoma cells in order to test their efficacy as immuno-stimulating adjuvant agents in inducing tumor-specific immunity. The transduction of the allogenic I-Ab alpha and beta chain genes into A20 cell resulted in a surface expression of the allogenic class II MHC molecules. The expression of the allogenic class II MHC antigen (I-Ab) in A20 cells enhanced the proliferation of T cells in a mixed lymphocyte tumor culture and in vitro cytotoxic T lymphocyte (CTL) generation against parental cells. The B7.1 gene, which is known to be a potent co-stimulatory molecule, was also transduced and expressed in A20 cells, either alone or in combination with I-Ab. The B7.1 transduction alone leads to a similar in vitro immune enhancing effect as I-Ab. When both the I-Ab and B7.1 genes were transduced, the in vitro immunostimulating capacity was further enhanced. Finally, we also tested the A20 cells that were transduced with I-Ab and/or B7.1 for their efficacy as preventive tumor vaccines in vivo. The results indicate that the A20 cells that express both the I-Ab and B7.1 have more potent vaccinating potential, compared to the cells that express only one of the molecules.
MeSH terms
AnimalsAntigens, CD80/genetics/*metabolismCancer Vaccines/genetics/immunologyGenes, MHC Class IIHistocompatibility Antigens Class II/*metabolismLymphocyte Culture Test, MixedLymphoma, B-Cell/genetics/*immunologyMiceMice, Inbred BALB CMice, Inbred C57BLNeoplasms, Experimental/immunology/therapyT-Lymphocytes/immunologyT-Lymphocytes, Cytotoxic/immunologyTransduction, GeneticTumor Cells, Cultured
PMID
11911464
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
장, 영주
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