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Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast.

Authors
Jae Yoo, E; Kyu Jang, Y; Ae Lee, M; Bjerling, P; Bum Kim, J; Ekwall, K; Hyun Seong, R; Dai Park, S
Citation
Biochemical and biophysical research communications, 295(4):970-974, 2002
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3(+) is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.
MeSH terms
Adenosine Triphosphatases/chemistry/*physiologyAmino Acid SequenceBlotting, WesternCell Nucleus/metabolismCell SurvivalChromatin/chemistry/metabolismDNA/metabolismDNA-Binding Proteins/chemistry/metabolism/*physiologyGene DeletionGene SilencingHeterochromatin/*metabolismHistones/metabolismMicroscopy, FluorescenceMolecular Sequence DataMutationPhenotypePhylogenyPrecipitin TestsProtein BindingProtein Structure, TertiarySchizosaccharomyces/*metabolismSequence Homology, Amino AcidTranscription, Genetic
PMID
12127990
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Journal Papers > School of Medicine / Graduate School of Medicine > Brain Science
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